PSW1, an LRR receptor kinase, regulates pod size in peanut

Pod size is a key agronomic trait that greatly determines peanut yield, the regulatory genes and molecular mechanisms that controlling peanut pod size are still unclear. Here, we used quantitative trait locus analysis to identify a peanut pod size regulator, POD SIZE/WEIGHT1 (PSW1), and characterize...

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Bibliographic Details
Published in:Plant biotechnology journal 2023-10, Vol.21 (10), p.2113-2124
Main Authors: Zhao, Kunkun, Wang, Long, Qiu, Ding, Cao, Zenghui, Wang, Kuopeng, Li, Zhan, Wang, Xiaoxuan, Wang, Jinzhi, Ma, Qian, Cao, Di, Qi, Yinyao, Zhao, Kai, Gong, Fangping, Li, Zhongfeng, Ren, Rui, Ma, Xingli, Zhang, Xingguo, Yu, Feng, Yin, Dongmei
Format: Article
Language:English
Subjects:
Agricultural production
Alleles
Cell division
Crop yield
Gene mapping
Genes
Genetic resources
Isoleucine
Kinases
Leucine
Molecular modelling
mRNA
Mutation
Peanuts
Peptides
Plant breeding
Plant species
Point mutation
Proteins
Quantitative trait loci
Receptors
Rice
Seeds
Stem cells
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Summary:Pod size is a key agronomic trait that greatly determines peanut yield, the regulatory genes and molecular mechanisms that controlling peanut pod size are still unclear. Here, we used quantitative trait locus analysis to identify a peanut pod size regulator, POD SIZE/WEIGHT1 (PSW1), and characterized the associated gene and protein. PSW1 encoded leucine-rich repeat receptor-like kinase (LRR-RLK) and positively regulated pod stemness. Mechanistically, this allele harbouring a 12-bp insertion in the promoter and a point mutation in the coding region of PSW1 causing a serine-to-isoleucine (S618I) substitution substantially increased mRNA abundance and the binding affinity of PSW1 for BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE 1 (BAK1). Notably, PSW1 (super-large pod allele of PSW1) expression led to up-regulation of a positive regulator of pod stemness PLETHORA 1 (PLT1), thereby resulting in larger pod size. Moreover, overexpression of PSW1 increased seed/fruit size in multiple plant species. Our work thus discovers a conserved function of PSW1 that controls pod size and provides a valuable genetic resource for breeding high-yield crops.
ISSN:1467-7644
1467-7652
1467-7652
DOI:10.1111/pbi.14117