MdNAC104 positively regulates apple cold tolerance via CBF-dependent and CBF-independent pathways

Low temperature is the main environmental factor affecting the yield, quality and geographical distribution of crops, which significantly restricts development of the fruit industry. The NAC (NAM, ATAF1/2 and CUC2) transcription factor (TF) family is involved in regulating plant cold tolerance, but...

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Bibliographic Details
Published in:Plant biotechnology journal 2023-10, Vol.21 (10), p.2057-2073
Main Authors: Mei, Chuang, Yang, Jie, Mei, Quanlin, Jia, Dongfeng, Yan, Peng, Feng, Beibei, Mamat, Aisajan, Gong, Xiaoqing, Guan, Qingmei, Mao, Ke, Wang, Jixun, Ma, Fengwang
Format: Article
Language:English
Subjects:
Accumulation
Anthocyanins
Antioxidants
Apples
Biosynthesis
Cold
Cold tolerance
Drought
Environmental factors
Enzymes
Flowers & plants
Gene expression
Gene regulation
Genes
Geographical distribution
Low temperature
Metabolomics
Osmoregulation
Physiology
Plant growth
Proteins
Reactive oxygen species
Regulatory mechanisms (biology)
Stress response
Temperature tolerance
Transcription factors
Transcriptomics
Transgenic plants
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Summary:Low temperature is the main environmental factor affecting the yield, quality and geographical distribution of crops, which significantly restricts development of the fruit industry. The NAC (NAM, ATAF1/2 and CUC2) transcription factor (TF) family is involved in regulating plant cold tolerance, but the mechanisms underlying these regulatory processes remain unclear. Here, the NAC TF MdNAC104 played a positive role in modulating apple cold tolerance. Under cold stress, MdNAC104-overexpressing transgenic plants exhibited less ion leakage and lower ROS (reactive oxygen species) accumulation, but higher contents of osmoregulatory substances and activities of antioxidant enzymes. Transcriptional regulation analysis showed that MdNAC104 directly bound to the MdCBF1 and MdCBF3 promoters to promote expression. In addition, based on combined transcriptomic and metabolomic analyses, as well as promoter binding and transcriptional regulation analyses, we found that MdNAC104 stimulated the accumulation of anthocyanin under cold conditions by upregulating the expression of anthocyanin synthesis-related genes, including MdCHS-b, MdCHI-a, MdF3H-a and MdANS-b, and increased the activities of the antioxidant enzymes by promoting the expression of the antioxidant enzyme-encoding genes MdFSD2 and MdPRXR1.1. In conclusion, this study revealed the MdNAC104 regulatory mechanism of cold tolerance in apple via CBF-dependent and CBF-independent pathways.
ISSN:1467-7644
1467-7652
1467-7652
DOI:10.1111/pbi.14112