An LC–MS/MS method for simultaneous determination of LB-100 and its active metabolite, endothall, in human plasma

We have developed and validated a novel LC–MS/MS method for the simultaneous quantification of LB-100 and its active metabolite, endothall, in human plasma following solid-phase extraction. LB-105 and endothall-D6 were used as internal standards. Chromatographic separation was achieved on a Hypercar...

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Bibliographic Details
Published in:Bioanalysis 2023-09, Vol.15 (17), p.1095-1107
Main Authors: Feng, Ye, Massarelli, Erminia, Forman, Eric, Kovach, John S, Salgia, Ravi, Synold, Timothy W
Format: Article
Language:English
Subjects:
chromatography
endothall
human plasma
LB-100
LC–MS
mass spectrometry
Methodology
oncology
pharmacokinetics
validation
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Summary:We have developed and validated a novel LC–MS/MS method for the simultaneous quantification of LB-100 and its active metabolite, endothall, in human plasma following solid-phase extraction. LB-105 and endothall-D6 were used as internal standards. Chromatographic separation was achieved on a Hypercarb™ column using 5 mM (NH ) CO and 30:70 (v/v) 100 mM (NH ) CO :acetonitrile as mobile phases. Detection was performed via positive electrospray ionization mode with multiple reaction monitoring. The assay exhibited linearity in the concentration range of 2.5–500 ng/ml for both analytes. Intra- and inter-assay precision and accuracy were within ±11%. LB-100 and endothall recoveries were 78.7 and 86.7%, respectively. The validated LC–MS/MS method enabled the accurate measurement of LB-100 and endothall in patient samples from an ongoing clinical trial ( ).
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2023-0078