Correlation Between Estrogen Receptor α Gene Polymorphism (c454-397T>C) with Serum Estradiol Levels and Known Risk Factors in Patients with Myocardial Infarction

• Published in: Indian journal of clinical biochemistry 2023-10, Vol.38 (4), p.495-504
• Main Authors: Aparna, R. R., Rajarajeswari, D., Prasad, M., Krishna, T. Sharmila, Ramalingam, K., Viswakumar, R., Fathima, Nusrath, Khan, Aleem Ahmed
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Biochemistry; Biomedical and Life Sciences; Chemistry/Food Science; Dyslipidemia; Estrogen receptors; Estrogens; Gene polymorphism; Genotyping; Heart attacks; Heterozygotes; High density lipoprotein; Life Sciences; Metabolic disorders; Microbiology; Morbidity; Myocardial infarction; Original; Original Research Article; Pathology; Polymorphism; Restriction fragment length polymorphism; Risk factors; Women
• ISSN: 0970-1915; 0974-0422
• DOI: 10.1007/s12291-022-01104-1

Myocardial infarction (MI) remains the most common cause of cardiac failure and continuous increasing rate of morbidity and mortality. We aimed to investigate the association of estrogen receptor-α (ESR1) gene polymorphism c454-397T>C with serum estradiol levels and dyslipidemia in 220 patients with MI in the age range of 35–70 years of both the genders. Genotyping study was performed through PCR-RFLP method using PvuII restriction enzyme. Serum estradiol level was estimated using the Access Sensitive Estradiol assay kit. Men patients had 43.2% increased risk for TC heterozygote in co-dominant (OR 10.66) and over-dominant models (OR 8.30), while women patients had 50% increased risk in co-dominant (OR 16.57) and over-dominant (OR 14.04) models. Variant C allele showed 25% increased risk of MI for in men (OR 2.24; CI 1.49–3.36; p  = 0.0001), and 24% increased risk in women (OR 3.35; CI 1.95–5.76; p  = 0.0001). Men patients had significantly increased serum estradiol levels compared to controls (25.28 ± 5.80 vs 17.04 ± 2.01; p