Spatial and temporal organization of the genome: Current state and future aims of the 4D nucleome project

The four-dimensional nucleome (4DN) consortium studies the architecture of the genome and the nucleus in space and time. We summarize progress by the consortium and highlight the development of technologies for (1) mapping genome folding and identifying roles of nuclear components and bodies, protei...

Full description

Saved in:
Bibliographic Details
Published in:Molecular cell 2023-08, Vol.83 (15), p.2624-2640
Main Authors: Dekker, Job, Alber, Frank, Aufmkolk, Sarah, Beliveau, Brian J, Bruneau, Benoit G, Belmont, Andrew S, Bintu, Lacramioara, Boettiger, Alistair, Calandrelli, Riccardo, Disteche, Christine M, Gilbert, David M, Gregor, Thomas, Hansen, Anders S, Huang, Bo, Huangfu, Danwei, Kalhor, Reza, Leslie, Christina S, Li, Wenbo, Li, Yun, Ma, Jian, Noble, William S, Park, Peter J, Phillips-Cremins, Jennifer E, Pollard, Katherine S, Rafelski, Susanne M, Ren, Bing, Ruan, Yijun, Shav-Tal, Yaron, Shen, Yin, Shendure, Jay, Shu, Xiaokun, Strambio-De-Castillia, Caterina, Vertii, Anastassiia, Zhang, Huaiying, Zhong, Sheng
Format: Article
Language:English
Subjects:
Biological Physics
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chromatin - metabolism
Genome - genetics
Physics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The four-dimensional nucleome (4DN) consortium studies the architecture of the genome and the nucleus in space and time. We summarize progress by the consortium and highlight the development of technologies for (1) mapping genome folding and identifying roles of nuclear components and bodies, proteins, and RNA, (2) characterizing nuclear organization with time or single-cell resolution, and (3) imaging of nuclear organization. With these tools, the consortium has provided over 2,000 public datasets. Integrative computational models based on these data are starting to reveal connections between genome structure and function. We then present a forward-looking perspective and outline current aims to (1) delineate dynamics of nuclear architecture at different timescales, from minutes to weeks as cells differentiate, in populations and in single cells, (2) characterize cis-determinants and trans-modulators of genome organization, (3) test functional consequences of changes in cis- and trans-regulators, and (4) develop predictive models of genome structure and function.
ISSN:1097-2765
1097-4164
1097-4164
DOI:10.1016/j.molcel.2023.06.018