Value of T6SS Core Gene hcp in Acinetobacter baumannii Respiratory Tract Infection

Hospital-acquired pneumonia caused by Acinetobacter baumannii is a major healthcare burden. Type VI Secretion System (T6SS) contributes to both virulence and drug resistance in this bacteria. This study aims to investigate the diagnostic value of hemolysin co-regulated protein (Hcp) gene in A. bauma...

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Bibliographic Details
Published in:Indian journal of microbiology 2023-09, Vol.63 (3), p.291-298
Main Authors: Hu, Yin-yin, Chen, Shuo, Zhang, Ya-dong, Lu, Qing-wen, Wang, Fei, Ren, Aijuan, Liu, Cai-xia
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Antimicrobial agents
Biomarkers
Biomedical and Life Sciences
Carbapenems
Diagnostic systems
Drug resistance
Hospitals
Inflammation
Interleukin 8
Life Sciences
Macrophages
Medical Microbiology
Microbiology
Original
Original Research Article
Pathogenicity
Pathogens
Pediatrics
Plasmids
Pneumonia
Polymerase chain reaction
Reagents
Respiratory tract
Respiratory tract infection
Virulence
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Summary:Hospital-acquired pneumonia caused by Acinetobacter baumannii is a major healthcare burden. Type VI Secretion System (T6SS) contributes to both virulence and drug resistance in this bacteria. This study aims to investigate the diagnostic value of hemolysin co-regulated protein (Hcp) gene in A. baumannii pneumonia and further explore the effect of hcp on clinical, pathogenicity and drug resistance. 53 clinical A. baumannii strains from patients’ respiratory tract at a teaching hospital were included in this study. Real-time quantitative polymerase chain reaction (qRT-PCR) was carried out to examine the expression of hcp . Recombinant Hcp expression plasmids (pET-28a(+)- hcp ) were constructed and his-tagged Hcp were purified to stimulate Tohoku Hospital Pediatrics-1 (THP-1) macrophages. Nuclear Factor Kappa B p65 (NF-κBp65) and Interleukin 8 (IL-8) were detected by qRT-PCR. Antimicrobial susceptibility testing (AST) were examined by an automated instrument system. Hcp gene had 92.6% sensitivity and 75% specificity for distinguishing invasive or colonizing A. baumannii from the respiratory tract. His-tagged Hcp induced NF-κBp65 and IL-8 at gene level in THP-1 macrophages. Additional, high hcp expression isolates showed higher rate of antimicrobial agent exposure (
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-023-01083-8