An overview of various methods for in vitro biofilm formation: a review

Biofilms are widely present in the natural environment and are difficult to remove as they are a survival strategy of microorganisms. Thus, the importance of studying biofilms is being increasingly recognized in food, medical, dental, and water quality-related industries. While research on biofilm d...

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Bibliographic Details
Published in:Food science and biotechnology 2023-10, Vol.32 (12), p.1617-1629
Main Authors: Han, Areum, Lee, Sun-Young
Format: Article
Language:English
Subjects:
Biofilms
Chemistry
Chemistry and Materials Science
Food Science
In vitro methods and tests
Invited Review
Microorganisms
Natural environment
Nutrition
Water quality
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Summary:Biofilms are widely present in the natural environment and are difficult to remove as they are a survival strategy of microorganisms. Thus, the importance of studying biofilms is being increasingly recognized in food, medical, dental, and water quality-related industries. While research on biofilm detection methods is actively progressing, research on biofilm formation is not progressing rapidly. Moreover, there are few standardized methods because biofilm formation is affected by various factors. However, comprehensive knowledge of biofilm formation is essential to select a suitable method for research purposes. To better understand the various in vitro biofilm formation methods, the principles and characteristics of each method are explained in this review by dividing the methods into static and dynamic systems. In addition, the applications of biofilm research based on various assays are also discussed.
ISSN:1226-7708
2092-6456
2092-6456
DOI:10.1007/s10068-023-01425-8