Per- and polyfluoroalkyl substances (PFAS) and thyroid hormone measurements in dried blood spots and neonatal characteristics: a pilot study

• Published in: Journal of exposure science & environmental epidemiology 2023-09, Vol.33 (5), p.737-747
• Main Authors: Rosen Vollmar, Ana K., Lin, Elizabeth Z., Nason, Sara L., Santiago, Katerina, Johnson, Caroline H., Ma, Xiaomei, Godri Pollitt, Krystal J., Deziel, Nicole C.
• Format: Article
• Language: English
• Subjects: Alkanesulfonic Acids; Blood; Chemical pollution; Child; Contaminants; Environmental Exposure; Environmental Pollutants - analysis; Environmental risk; Epidemiology; Exposure; Fluorocarbons; High performance liquid chromatography; Hormones; Humans; Infant, Newborn; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medical screening; Medicine; Medicine & Public Health; Neonates; Pediatrics; Perfluoroalkyl & polyfluoroalkyl substances; Perfluorochemicals; Perfluorooctane sulfonic acid; Perfluorooctanoic acid; Pilot Projects; Population studies; Population-based studies; Risk factors; Thyroid; Thyroid diseases; Thyroid gland; Thyroid Hormones
• ISSN: 1559-0631; 1559-064X
• DOI: 10.1038/s41370-023-00603-4

Background Pediatric thyroid diseases have been increasing in recent years. Environmental risk factors such as exposures to chemical contaminants may play a role but are largely unexplored. Archived neonatal dried blood spots (DBS) offer an innovative approach to investigate environmental exposures and effects. Objective In this pilot study, we applied a new method for quantifying per- and polyfluoroalkyl substances (PFAS) to 18 archived DBS from babies born in California from 1985–2018 and acquired thyroid hormone measurements from newborn screening tests. Leveraging these novel data, we evaluated (1) changes in the concentrations of eight PFAS over time and (2) the relationship between PFAS concentrations, thyroid hormone concentrations, and neonatal characteristics to inform future research. Methods PFAS concentrations in DBS were measured using ultra-high-performance liquid chromatography-mass spectrometry. Summary statistics and non-parametric Wilcoxon rank-sum and Kruskal–Wallis tests were used to evaluate temporal changes in PFAS concentrations and relationships between PFAS concentrations, thyroid hormone concentrations, and neonatal characteristics. Results The concentration and detection frequencies of several PFAS (PFOA, PFOS, and PFOSA) declined over the assessment period. We observed that the timing of specimen collection in hours after birth was related to thyroid hormone but not PFAS concentrations, and that thyroid hormones were related to some PFAS concentrations (PFOA and PFOS). Impact statement This pilot study examines the relationship between concentrations of eight per- and polyfluoroalkyl substances (PFAS), thyroid hormone levels, and neonatal characteristics in newborn dried blood spots (DBS) collected over a period of 33 years. To our knowledge, 6 of the 22 PFAS we attempted to measure have not been quantified previously in neonatal DBS, and this is the first study to examine both PFAS and thyroid hormone concentrations using DBS. This research demonstrates the feasibility of using newborn DBS for quantifying PFAS exposures in population-based studies, highlights methodological considerations in the use of thyroid hormone data for future studies using newborn DBS, and indicates potential relationships between PFAS concentrations and thyroid hormones for follow-up in future research.