Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

• Published in: Antonie van Leeuwenhoek 2023-11, Vol.116 (11), p.1209-1225
• Main Authors: Godinho, Ofélia, Klimek, Dominika, Jackiewicz, Adrianna, Guedes, Bárbara, Almeida, Eduarda, Calisto, Rita, Vitorino, Inês Rosado, Santos, José Diogo Neves, González, Ignacio, Lobo-da-Cunha, Alexandre, Calusinska, Magdalena, Quinteira, Sandra, Lage, Olga Maria
• Format: Article
• Language: English
• Subjects: Amino acids; Biomedical and Life Sciences; Brackish water; Deoxyribonucleic acid; Dietary supplements; DNA; DNA sequencing; DNA-directed RNA polymerase; Genomes; Leukocytes (neutrophilic); Life Sciences; Medical Microbiology; Microbiology; N-Acetylglucosamine; New species; Nucleotide sequence; Nucleotides; Original Paper; Phylogenetics; Phylogeny; Planctomycetota; Plant Sciences; Rivers; RNA polymerase; RpoB protein; rRNA 16S; Soil Science & Conservation; Water analysis; Water sampling
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-023-01877-2

A bacterial strain was isolated from a brackish water sample of Tagus river, Alcochete, Portugal and was designated TO1_6 T . It forms light pink colonies on M13 medium supplemented with N -acetylglucosamine. Cells are pear-shaped to spherical, form rosettes and divide by budding. Strain TO1_6 T presents a mesophilic and neutrophilic profile, with optimum growth at 20 to 25 °C and pH 7.0 to 7.5, and vitamin supplementation is not required to promote its growth. The genome of the novel isolate is 7.77 Mbp in size and has a DNA G + C content of 56.3%. Based on its 16S rRNA gene sequence, this strain is affiliated with the phylum Planctomycetota . Further taxonomic characterization using additional phylogenetic markers, namely rpoB gene sequence (encoding the β-subunit of the DNA-dependent RNA polymerase), as well as Percentage of conserved proteins, average nucleotide identity and average amino acid identity, suggest the affiliation of strain TO1_6 T to the genus Stieleria , a recently described taxon in the family Pirellulaceae, order Pirellulales and class Planctomycetia . Based on the genotypic, phylogenetic and physiological characterization, we here describe a new species represented by the type strain TO1_6 T (= CECT 30432 T , = LMG 32465 T ), for which the name Stieleria tagensis sp. nov. is proposed.