Chromosome-level genome of the venomous snail Kalloconus canariensis: a valuable model for venomics and comparative genomics

Abstract Background Genomes are powerful resources to understand the evolutionary mechanisms underpinning the origin and diversification of the venoms of cone snails (Conidae: Caenogastropoda) and could aid in the development of novel drugs. Findings Here, we used PacBio continuous long reads and Om...

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Bibliographic Details
Published in:Gigascience 2023-01, Vol.12
Main Authors: Herráez-Pérez, Ana, Pardos-Blas, José Ramón, Afonso, Carlos M L, Tenorio, Manuel J, Zardoya, Rafael
Format: Article
Language:English
Subjects:
Animals
Annotations
Caenogastropoda
Chromosomes
Chromosomes - genetics
Comparative analysis
Conidae
Data Note
Drug development
Evolutionary genetics
Gastropoda
Gene duplication
Genomes
Genomic analysis
Genomics
Mollusks
Peptides
Phylogeny
Snails
Snails - genetics
Venoms
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Summary:Abstract Background Genomes are powerful resources to understand the evolutionary mechanisms underpinning the origin and diversification of the venoms of cone snails (Conidae: Caenogastropoda) and could aid in the development of novel drugs. Findings Here, we used PacBio continuous long reads and Omni-C data to assemble the chromosome-level genome of Kalloconus canariensis, a vermivorous cone endemic to the Canary Islands. The final genome size was 2.87 Gb, with a N50 of 79.75 Mb and 91% of the reads located into the 35 largest scaffolds. Up to 55.80% of the genome was annotated as repetitive regions, being class I of transposable elements (16.65%) predominant. The annotation estimated 34,287 gene models. Comparative analysis of this genome with the 2 cone snail genomes released to date (Dendroconus betulinus and Lautoconus ventricosus) revealed similar genome sizes and organization, although chromosome sizes tended to be shorter in K. canariensis. Phylogenetic relationships within subclass Caenogastropoda were recovered with strong statistical support. The family Conidae was recovered as a clade, with K. canariensis plus L. ventricosus sister to D. betulinus. Conclusions Despite the great diversity of cone snails (>900 species) and their venoms (hundreds of peptides per species), only 2 recently reported genomes are available for the group. The high-quality chromosome-level assembly of K. canariensis will be a valuable reference for studying the origin and evolution of conotoxin genes as well as whole-genome duplication events during gastropod evolution.
ISSN:2047-217X
2047-217X
DOI:10.1093/gigascience/giad075