On-line clearing and staining method for the efficient optical imaging of large volume samples at the cellular resolution

Optical microscopy is a powerful tool for exploring the structure and function of organisms. However, the three-dimensional (3D) imaging of large volume samples is time-consuming and difficult. In this manuscript, we described an on-line clearing and staining method for efficient imaging of large vo...

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Published in:Biomedical optics express 2023-09, Vol.14 (9), p.4800-4813
Main Authors: Zhang, Yunfei, Liu, Guangcai, Li, Xiangning, Gong, Hui, Luo, Qingming, Yang, Xiaoquan
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creator Zhang, Yunfei
Liu, Guangcai
Li, Xiangning
Gong, Hui
Luo, Qingming
Yang, Xiaoquan
description Optical microscopy is a powerful tool for exploring the structure and function of organisms. However, the three-dimensional (3D) imaging of large volume samples is time-consuming and difficult. In this manuscript, we described an on-line clearing and staining method for efficient imaging of large volume samples at the cellular resolution. The optimized cocktail can increase staining and imaging depth to reduce the sectioning and scanning time, more than doubling the operational efficiency of the system. Using this method, we demonstrated the rapid acquisition of Aβ plaques in whole mouse brain and obtained a complete set of cytoarchitecture images of an adult porcine hemisphere at 1.625 × 1.625 × 10 µm 3 voxel resolution for about 49 hours.
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title On-line clearing and staining method for the efficient optical imaging of large volume samples at the cellular resolution
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