Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) reveals potential lipid markers between infrapatellar fat pad biopsies of osteoarthritis and cartilage defect patients

• Published in: Analytical and bioanalytical chemistry 2023-10, Vol.415 (24), p.5997-6007
• Main Authors: Haartmans, Mirella J. J., Claes, Britt S. R., Eijkel, Gert B., Emanuel, Kaj S., Tuijthof, Gabrielle J. M., Heeren, Ron M. A., Emans, Pieter J., Cillero-Pastor, Berta
• Format: Article
• Language: English
• Subjects: Adipose Tissue - pathology; Aged; Analysis; Analytical Chemistry; Arachidonic acid; Arthritis; Biochemistry; Biological markers; Biomarkers; Biopsy; Cartilage; Cartilage - pathology; Cartilage diseases; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Connective tissue; Connective tissues; Defects; Desorption; Food Science; Heterogeneity; Humans; Inflammation; Ionization; Ions; Laboratory Medicine; Lasers; Lipids; Mass spectrometry; Mass spectroscopy; Medical imaging; Methods; Monitoring/Environmental Analysis; Osteoarthritis; Osteoarthritis, Knee - diagnostic imaging; Osteoarthritis, Knee - pathology; Phosphatidylethanolamine; Phospholipids; Research Paper; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
• ISSN: 1618-2642; 1618-2650; 1618-2650
• DOI: 10.1007/s00216-023-04871-9

The incidence of osteoarthritis (OA) has been expected to increase due to an aging population, as well as an increased incidence of intra-articular (osteo-) chondral damage. Lipids have already been shown to be involved in the inflammatory process of OA. This study aims at revealing region-specific lipid profiles of the infrapatellar fat pad (IPFP) of OA or cartilage defect patients by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which could be used as biomarkers for early OA detection. A higher presence of phospholipids was found in OA patients compared with cartilage defect patients. In addition, a higher abundance of ether-linked phosphatidylethanolamines (PE O-s) containing arachidonic acid was specifically found in OA patients compared with cartilage defect patients. These lipids were mainly found in the connective tissue of the IPFP. Specific lipid species were associated to OA patients compared with cartilage defect patients. PE O-s have been suggested as possible biomarkers for OA. As these were found more abundantly in the connective tissue, the IPFP’s intra-tissue heterogeneity might play an important role in biomarker discovery, implying that the amount of fibrous tissue is associated with OA. Graphical Abstract