Axonal transport of Frizzled5 by Alcadein α-containing vesicles is associated with kinesin-1

• Published in: Molecular biology of the cell 2023-10, Vol.34 (11), p.ar110-ar110
• Main Authors: Shiraki, Yuzuha, Mitsuma, Monet, Takada, Ritsuko, Hata, Saori, Kitamura, Akira, Takada, Shinji, Kinjo, Masataka, Taru, Hidenori, Müller, Ulrike C., Yamamoto, Tohru, Sobu, Yuriko, Suzuki, Toshiharu
• Format: Article
• Language: English
• ISSN: 1059-1524; 1939-4586
• DOI: 10.1091/mbc.E22-10-0495

Alcadein α (Alcα) and amyloid-β protein precursor (APP) are cargo receptors that associate vesicles with kinesin-1. These vesicles, which contain either Alcα or APP, transport various proteins/cargo molecules into axon nerve terminals. Here, we analyzed immune-isolated Alcα- and APP-containing vesicles of adult mouse brains with LC–MS/MS and identified proteins present in vesicles that contained either Alcα or APP. Among these proteins, Frizzled-5 (Fzd5), a Wnt receptor, was detected mainly in Alcα vesicles. Although colocalization ratios of Fzd5 with Alcα are low in the neurites of differentiating neurons by a low expression of Fzd5 in embryonic brains, the suppression of Alcα expression decreased the localization of Fzd5 in neurites of primary cultured neurons. Furthermore, Fzd5-EGFP expressed in primary cultured neurons was preferentially transported in axons with the transport velocities of Alcα vesicles. In synaptosomal fractions of adult-mice brains that express higher levels of Fzd5, the amount of Fzd5 and the phosphorylation level of calcium/calmodulin-dependent protein kinase-II were reduced in the Alcα-deficient mice. These results suggest that reduced transport of Fzd5 by Alcα-containing vesicles associated with kinesin-1 in axon terminals may impair the response to Wnt ligands in the noncanonical Ca 2+ -dependent signal transduction pathway at nerve terminals of mature neurons. Alcadein α/Alcα is a cargo receptor that associates vesicles with kinesin-1. Alcα vesicles include characteristic proteins in adult brain neurons. Lack of Alcα impairs signal transduction at nerve terminals.