The simultaneous determination of carbon dioxide release and oxygen uptake in suspensions of plant leaf mitochondria oxidizing glycine

The construction and operation of a device for continuous measurement of CO2 release by suspensions of respiring mitochondria is described. A combination of this device with a Clark-type O2 electrode was used for simultaneous measurement of respiration and of CO2 release by spinach and pea leaf mito...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1987-02, Vol.83 (2), p.349-353
Main Authors: Lilley, R.M, Ebbighausen, H, Heldt, H.W
Format: Article
Language:English
Subjects:
Biological and medical sciences
CARBON DIOXIDE
Cell structures and functions
Chloroplasts
Decarboxylation
DIOXIDO DE CARBONO
DIOXYDE DE CARBONE
DISPERSION
DISPERSIONS
Electrodes
ETAT DISPERSE
FEUILLE
Fundamental and applied biological sciences. Psychology
GLICINA
GLYCINE
HOJAS
LEAVES
MEASUREMENT
MEDICION
Membranes and Bioenergetics
MESURE
MITOCHONDRIA
Mitochondria and cell respiration
MITOCHONDRIE
MITOCONDRIA
Molecular and cellular biology
OXIDACION
OXIDATION
OXYDATION
Peas
PISUM SATIVUM
Plants
RESPIRACION
RESPIRATION
Spinach
SPINACIA OLERACEA
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Description
Summary:The construction and operation of a device for continuous measurement of CO2 release by suspensions of respiring mitochondria is described. A combination of this device with a Clark-type O2 electrode was used for simultaneous measurement of respiration and of CO2 release by spinach and pea leaf mitochondria with glycine as substrate. Both mitochondrial preparations showed high rates of respiration and high respiratory control ratios. The addition of oxaloacetate not only inhibited O2 uptake substantially, but also greatly stimulated glycine oxidation as monitored by CO2 release. In spinach leaf mitochondria, the maximal rates of glycine oxidation thus obtained, were two times higher than the rate of glycine oxidation required at average rates of photorespiration. It is concluded from these results that under saturating conditions the capacity of glycine oxidation by intact mitochondria exceeds the capacity of glycine-dependent respiration.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.83.2.349