World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses

Long-term modelization of cancer as it changes in the human body is a difficult goal, particularly when designing and testing new therapeutic strategies. This becomes even more difficult with metastasis modeling to show chemotherapeutic molecule delivery directly to tumoral cells. Advanced therapeut...

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Bibliographic Details
Published in:Cancers 2023-09, Vol.15 (19), p.4724
Main Authors: McGuckin, Colin, Forraz, Nico, Milet, Clément, Lacroix, Mathieu, Sbirkov, Yordan, Sarafian, Victoria, Ebel, Caroline, Spindler, Anita, Koerper, Véronique, Balloul, Jean-Marc, Quéméneur, Eric, Zaupa, Cécile
Format: Article
Language:English
Subjects:
3-D printers
Additive manufacturing
Analysis
Biobanks
Cancer
Cancer research
Cancer therapies
Cell culture
Chemotherapy
Colorectal cancer
Colorectal carcinoma
Costs
Drug delivery
Fibroblasts
Health aspects
Medical research
Medical screening
Metastases
Metastasis
Mutagenesis
Oncology, Experimental
Oncolysis
Pharmacogenetics
Precision medicine
Tumors
Viruses
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Summary:Long-term modelization of cancer as it changes in the human body is a difficult goal, particularly when designing and testing new therapeutic strategies. This becomes even more difficult with metastasis modeling to show chemotherapeutic molecule delivery directly to tumoral cells. Advanced therapeutics, including oncolytic viruses, antibody-based and cell-based therapies are increasing. The question is, are screening tests also evolving? Next-generation therapeutics need equally advanced screening tests, which whilst difficult to achieve, are the goal of our work here, creating models of micro- and macrotumors using 3D bioprinting. We developed advanced colorectal cancer tumor processing techniques to provide options for cellular expansion, microtumor printing, and long-term models, which allow for the evaluation of the kinetics of penetration testing, therapeutic success, targeted therapies, and personalized medicine. We describe how we tested tumors from a primary colorectal patient and, applying 3D bioprinting, matured long-term models for oncolytic metastatic screening. Three-dimensional microtumors were kept alive for the longest time ever recorded in vitro, allowing longitudinal studies, screening of oncolytic viruses and realistic modelization of colorectal cancer. These 3D bioprinted models were maintained for around 6 months and were able to demonstrate the effective delivery of a product to the tumoral environment and represent a step forward in therapeutic screening.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers15194724