Assessment of selected muscle damage markers and zonulin concentration after maximum-intensity exercise in men with type 1 diabetes treated with a personal insulin pump

• Published in: Acta diabetologica 2023-12, Vol.60 (12), p.1675-1683
• Main Authors: Matejko, Bartłomiej, Tota, Łukasz, Morawska-Tota, Małgorzata, Pałka, Tomasz, Malecki, Maciej T., Klupa, Tomasz
• Format: Article
• Language: English
• Subjects: Body fat; Body mass index; Cortisol; Dehydrogenases; Diabetes; Diabetes mellitus (insulin dependent); Exercise; Hemoglobin; Hormones; Insulin; Internal Medicine; Intestinal microflora; Intestine; L-Lactate dehydrogenase; Lactic acid; Medicine; Medicine & Public Health; Metabolic Diseases; Myoglobins; Original; Original Article; Physical activity; Physical fitness; Physical training; Testosterone; Vitamin D
• ISSN: 1432-5233; 0940-5429; 1432-5233
• DOI: 10.1007/s00592-023-02157-1

Aim Exercise-induced muscle damage depends on exercise intensity and duration and on individual susceptibility. Mechanical and metabolic stress may disturb the intestinal microflora. The study evaluated selected muscle damage markers and zonulin concentration after maximum-intensity exercise in type 1 diabetes (T1D) men compared with healthy controls. Methods The study involved 16 T1D participants and 28 controls matched by age (22.7 [21.3–25.1] vs. 22.6 [20.9–26.3] years), body mass index (24.2 ± 1.6 vs. 24.2 ± 1.9 kg/m 2 ), and body fat percentage (16.1 ± 5.2 vs. 14.9 ± 4.6%). The T1D group had 11.3 ± 5.1 years of diabetes duration and a suboptimal mean glycated haemoglobin level of 7.2 ± 1.1%. The subjects underwent a graded running treadmill test until exhaustion. Lactate concentration was assessed in arterialized blood at baseline and 3 and 20 min after the test. Cortisol, testosterone, tumour necrosis factor α, myoglobin, lactate dehydrogenase, zonulin, and vitamin D levels were evaluated in cubital fossa vein blood before and 60 min after the test. Results T1D patients presented higher baseline zonulin, myoglobin concentration, testosterone/cortisol ratio, and lower maximal oxygen uptake. On adjusting for the baseline values, the groups differed in zonulin, lactate dehydrogenase, and myoglobin levels, testosterone/cortisol ratio, and lactate concentration determined 20 min after exercise ( P