A gravity-driven droplet fluidic point-of-care test

We report a simple droplet fluidic point-of-care test (POCT) that uses gravity to manipulate the sequence, timing, and motion of droplets on a surface. To fabricate this POCT, we first developed a surface coating toolbox of nine different coatings with three levels of wettability and three levels of...

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Bibliographic Details
Published in:Device 2023-07, Vol.1 (1)
Main Authors: Vahabi, Hamed, Liu, Jason, Dai, Yifan, Joh, Daniel Y, Britton, Rhett, Heggestad, Jacob, Kinnamon, David, Rajput, Satyam, Chilkoti, Ashutosh
Format: Article
Language:English
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Summary:We report a simple droplet fluidic point-of-care test (POCT) that uses gravity to manipulate the sequence, timing, and motion of droplets on a surface. To fabricate this POCT, we first developed a surface coating toolbox of nine different coatings with three levels of wettability and three levels of slipperiness that can be independently tailored. We then fabricated a device that has interconnected fluidic elements-pumps, flow resistors and flow guides-on a highly slippery solid surface to precisely control the timing and sequence of motion of multiple droplets and their interactions on the surface. We then used this device to carry out a multi-step enzymatic assay of a clinically relevant analyte-lactate dehydrogenase (LDH)-to demonstrate the application of this technology for point-of-care diagnosis.
ISSN:2666-9986
2666-9994
2666-9986
DOI:10.1016/j.device.2023.100009