Class I Biocompatible DLP-Printed Acrylate Impairs Adhesion and Proliferation of Human Mesenchymal Stromal Cells in Indirect Cytotoxicity Assay

The popular method of digital light processing 3D printing (DLP) for complex and individual laboratory equipment requires materials that are as inert as possible for use in contact with cells for subsequent investigations. However, the per se incomplete curing of acrylate resins by UV light leaves r...

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Bibliographic Details
Published in:BioMed research international 2023, Vol.2023 (1), p.8305995
Main Authors: Alt, Franziska, Heinemann, Christiane, Kruppke, Benjamin
Format: Article
Language:English
Subjects:
3-D printers
Acrylates - pharmacology
Adherent cells
Adhesion
Biocompatibility
Bone marrow
Cell adhesion
Cell culture
Cell Proliferation
Crosslinking
Cytotoxicity
Deoxyribonucleic acid
DNA
Humans
Inserts
Medical research
Mesenchymal Stem Cells
Polymerization
Polystyrene
Polystyrene resins
Printing, Three-Dimensional
Resins
Resins, Plant
Stromal cells
Surgical instruments
Three dimensional printing
Toxicity
Toxicity testing
Ultraviolet radiation
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Summary:The popular method of digital light processing 3D printing (DLP) for complex and individual laboratory equipment requires materials that are as inert as possible for use in contact with cells for subsequent investigations. However, the per se incomplete curing of acrylate resins by UV light leaves residuals that are not suitable for cell culture application. Therefore, we evaluated the cytotoxicity of four commercially available acrylate resins with bone marrow-derived human mesenchymal stromal cells (BM-hMSC) in an indirect cytotoxicity test. This involved incubating the printed cylinders in Transwell™ inserts for 7 days. While the degree of crosslinking did not increase significantly between freshly printed and stored samples (3 weeks in ambient conditions), the storage improved the material’s performance in terms of cytocompatibility. The DNA amount and LDH activity showed a direct influence of the resin residuals on cell adhesion. The class I acrylate Surgical Guide™ left no adherent cells after 7 days, regardless of previous storage. In comparison, the Basic Ivory™ resin after storage allowed same amount of adherent cells after 7 days as the polystyrene reference. We conclude that resin residuals of certain materials are released, which allows the use of the resins in indirect contact with cells thereafter.
ISSN:2314-6133
2314-6141
2314-6141
DOI:10.1155/2023/8305995