The promoting effects of GPR176 expression on proliferation, chemoresistance, lipogenesis and invasion of oesophageal cancer

• Published in: Journal of cancer research and clinical oncology 2023-11, Vol.149 (16), p.14641-14655
• Main Authors: Yun, Wen-jing, Li, Jun, Yin, Nan-chang, Zhang, Cong-yu, Cui, Zheng-guo, Zhang, Li, Zheng, Hua-chuan
• Format: Article
• Language: English
• Subjects: Actin; Apoptosis; Bioinformatics; Cancer; Cancer Research; Carcinogenesis; Cell Line, Tumor; Cell Proliferation; Chemoresistance; Drug Resistance, Neoplasm - genetics; Endoplasmic reticulum; Esophageal cancer; Esophageal Neoplasms - drug therapy; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophagus; Extracellular matrix; G protein-coupled receptors; Gene expression; Gene Expression Regulation, Neoplastic; Genes; Hematology; Humans; Internal Medicine; Lipids; Lipogenesis; Medicine; Medicine & Public Health; Molecular modelling; Myosin; Oncology; Oxidoreductase; Proteins; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; RNA, Messenger - genetics
• ISSN: 0171-5216; 1432-1335; 1432-1335
• DOI: 10.1007/s00432-023-05256-2

Purpose As a member of the G-protein-coupled receptor 1 family, the G-protein-coupled receptor 176 (GPR176) gene encodes a glycosylated protein made up of 515 amino acids. The current study was performed to evaluate the impact of GPR176 on the clinicopathology and prognosis of oesophageal cancer, as well as uncover its molecular mechanisms. Methods Bioinformatics and clinical tissue samples were used to detect the expression and clinicopathological significance of GPR176 in oesophageal cancer. The expression, proliferation, migration and invasion, apoptosis and lipid droplet formation of GPR176 gene in oesophageal cancer were performed as phenotypic readouts. Results Here, RT-PCR and bioinformatic analyses revealed that GPR176 mRNA expression was significantly higher in oesophageal cancer than in normal mucosa (p