Evaluation of potential aging biomarkers in healthy individuals: telomerase, AGEs, GDF11/15, sirtuin 1, NAD+, NLRP3, DNA/RNA damage, and klotho

• Published in: Biogerontology (Dordrecht) 2023-12, Vol.24 (6), p.937-955
• Main Authors: Borsky, Pavel, Holmannova, Drahomira, Andrys, Ctirad, Kremlacek, Jan, Fiala, Zdenek, Parova, Helena, Rehacek, Vit, Svadlakova, Tereza, Byma, Svatopluk, Kucera, Otto, Borska, Lenka
• Format: Article
• Language: English
• Subjects: Age; Aged; Aging; Aging - genetics; Biomarkers; Biomedical and Life Sciences; Bone Morphogenetic Proteins; Cell Biology; Cognitive ability; Developmental Biology; DNA; DNA damage; Female; Females; Geriatrics; Geriatrics/Gerontology; Glycation End Products, Advanced; Growth differentiation factor 11; Growth Differentiation Factors - metabolism; Health Status; Humans; Inflammasomes; Klotho protein; Life Sciences; Male; NAD; NLR Family, Pyrin Domain-Containing 3 Protein; Research Article; Sex; Sirtuin 1; Telomerase
• ISSN: 1389-5729; 1573-6768; 1573-6768
• DOI: 10.1007/s10522-023-10054-x

Aging is a natural process of gradual decrease in physical and mental capacity. Biological age (accumulation of changes and damage) and chronological age (years lived) may differ. Biological age reflects the risk of various types of disease and death from any cause. We selected potential biomarkers of aging - telomerase, AGEs, GDF11 and 15 (growth differentiation factor 11/15), sirtuin 1, NAD + (nicotinamide adenine dinucleotide), inflammasome NLRP3, DNA/RNA damage, and klotho to investigate changes in their levels depending on age and sex. We included 169 healthy volunteers and divided them into groups according to age (under 35; 35–50; over 50) and sex (male, female; male and female under 35; 35–50, over 50). Markers were analyzed using commercial ELISA kits. We found differences in values depending on age and gender. GDF15 increased with age (under 30 and 35–50 p