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Evidence that a new antibiotic flavone glycoside chemically defends the sea grass Thalassia testudinum against zoosporic fungi
Significantly fewer thraustochytrid protists (zoosporic fungi) were observed in association with healthy leaf tissue of the marine angiosperm Thalassia testudinum than in association with sterilized samples that were returned to the collection site for 48 h. In support of the hypothesis that sea gra...
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| Published in: | Applied and environmental microbiology 1998-04, Vol.64 (4), p.1490-1496 |
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| container_title | Applied and environmental microbiology |
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| creator | Jensen, P.R. (University of California-San Diego, La Jolla, CA.) Jenkins, K.M Porter, D Fenical, W |
| description | Significantly fewer thraustochytrid protists (zoosporic fungi) were observed in association with healthy leaf tissue of the marine angiosperm Thalassia testudinum than in association with sterilized samples that were returned to the collection site for 48 h. In support of the hypothesis that sea grass secondary metabolites were responsible for these differences, extracts of healthy T. testudinum leaf tissues inhibited the growth of the co-occurring thraustochytrid Schizochytrium aggregatum and deterred the attachment of S. aggregatum motile zoospores to an extract-impregnated substrate. By using S. aggregatum for bioassay-guided chemical fractionation, a new flavone glycoside was isolated and structurally characterized as luteolin 7-O-beta-D-glucopyranosyl-2"-sulfate. Whole-leaf tissue concentrations of this metabolite (4 mg/ml of wet leaf tissue) inhibited S. aggregatum attachment, and a significantly lower concentration (270 micrograms/ml) reduced thraustochytrid growth by 50%, suggesting that natural concentrations are at least 15 times greater than that needed for significant microbiological effects. These results offer the first complete chemical characterization of a sea grass sulfated flavone glycoside and provide evidence that a secondary metabolite chemically defends T. testudinum against fouling microorganisms |
| doi_str_mv | 10.1128/aem.64.4.1490-1496.1998 |
| format | article |
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(University of California-San Diego, La Jolla, CA.) ; Jenkins, K.M ; Porter, D ; Fenical, W</creator><creatorcontrib>Jensen, P.R. (University of California-San Diego, La Jolla, CA.) ; Jenkins, K.M ; Porter, D ; Fenical, W</creatorcontrib><description>Significantly fewer thraustochytrid protists (zoosporic fungi) were observed in association with healthy leaf tissue of the marine angiosperm Thalassia testudinum than in association with sterilized samples that were returned to the collection site for 48 h. In support of the hypothesis that sea grass secondary metabolites were responsible for these differences, extracts of healthy T. testudinum leaf tissues inhibited the growth of the co-occurring thraustochytrid Schizochytrium aggregatum and deterred the attachment of S. aggregatum motile zoospores to an extract-impregnated substrate. By using S. aggregatum for bioassay-guided chemical fractionation, a new flavone glycoside was isolated and structurally characterized as luteolin 7-O-beta-D-glucopyranosyl-2"-sulfate. Whole-leaf tissue concentrations of this metabolite (4 mg/ml of wet leaf tissue) inhibited S. aggregatum attachment, and a significantly lower concentration (270 micrograms/ml) reduced thraustochytrid growth by 50%, suggesting that natural concentrations are at least 15 times greater than that needed for significant microbiological effects. These results offer the first complete chemical characterization of a sea grass sulfated flavone glycoside and provide evidence that a secondary metabolite chemically defends T. testudinum against fouling microorganisms</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.64.4.1490-1496.1998</identifier><identifier>PMID: 16349549</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ANGIOSPERMAS ; ANGIOSPERME ; ANGIOSPERMS ; Animal, plant and microbial ecology ; ANTIBIOTIC PROPERTIES ; ANTIBIOTICOS ; ANTIBIOTICS ; ANTIBIOTIQUE ; Biological and medical sciences ; CHAMPIGNON ; DEFENCE MECHANISMS ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; FUNGI ; General Microbial Ecology ; GLICOSIDOS ; GLYCOSIDE ; GLYCOSIDES ; Grasses ; HONGOS ; Marine ; MECANISME DE DEFENSE ; MECANISMOS DE DEFENSA ; Microbial ecology ; Microbiology ; Microflora of plants ; Pathology, epidemiology, host-fungus relationships. Damages, economic importance ; Phytopathology. Animal pests. Plant and forest protection ; PROPIEDADES ANTIBIOTICAS ; PROPRIETE ANTIBIOTIQUE ; Thalassia testudinum</subject><ispartof>Applied and environmental microbiology, 1998-04, Vol.64 (4), p.1490-1496</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Apr 1998</rights><rights>Copyright © 1998, American Society for Microbiology 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-9b423688391c4a16024f182781726209b7fe6b1de8c7ca20bb3948a0e3c7f88f3</citedby><cites>FETCH-LOGICAL-c544t-9b423688391c4a16024f182781726209b7fe6b1de8c7ca20bb3948a0e3c7f88f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC106175/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC106175/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2259995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16349549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jensen, P.R. (University of California-San Diego, La Jolla, CA.)</creatorcontrib><creatorcontrib>Jenkins, K.M</creatorcontrib><creatorcontrib>Porter, D</creatorcontrib><creatorcontrib>Fenical, W</creatorcontrib><title>Evidence that a new antibiotic flavone glycoside chemically defends the sea grass Thalassia testudinum against zoosporic fungi</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Significantly fewer thraustochytrid protists (zoosporic fungi) were observed in association with healthy leaf tissue of the marine angiosperm Thalassia testudinum than in association with sterilized samples that were returned to the collection site for 48 h. In support of the hypothesis that sea grass secondary metabolites were responsible for these differences, extracts of healthy T. testudinum leaf tissues inhibited the growth of the co-occurring thraustochytrid Schizochytrium aggregatum and deterred the attachment of S. aggregatum motile zoospores to an extract-impregnated substrate. By using S. aggregatum for bioassay-guided chemical fractionation, a new flavone glycoside was isolated and structurally characterized as luteolin 7-O-beta-D-glucopyranosyl-2"-sulfate. Whole-leaf tissue concentrations of this metabolite (4 mg/ml of wet leaf tissue) inhibited S. aggregatum attachment, and a significantly lower concentration (270 micrograms/ml) reduced thraustochytrid growth by 50%, suggesting that natural concentrations are at least 15 times greater than that needed for significant microbiological effects. 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(University of California-San Diego, La Jolla, CA.)</au><au>Jenkins, K.M</au><au>Porter, D</au><au>Fenical, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence that a new antibiotic flavone glycoside chemically defends the sea grass Thalassia testudinum against zoosporic fungi</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>64</volume><issue>4</issue><spage>1490</spage><epage>1496</epage><pages>1490-1496</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Significantly fewer thraustochytrid protists (zoosporic fungi) were observed in association with healthy leaf tissue of the marine angiosperm Thalassia testudinum than in association with sterilized samples that were returned to the collection site for 48 h. In support of the hypothesis that sea grass secondary metabolites were responsible for these differences, extracts of healthy T. testudinum leaf tissues inhibited the growth of the co-occurring thraustochytrid Schizochytrium aggregatum and deterred the attachment of S. aggregatum motile zoospores to an extract-impregnated substrate. By using S. aggregatum for bioassay-guided chemical fractionation, a new flavone glycoside was isolated and structurally characterized as luteolin 7-O-beta-D-glucopyranosyl-2"-sulfate. Whole-leaf tissue concentrations of this metabolite (4 mg/ml of wet leaf tissue) inhibited S. aggregatum attachment, and a significantly lower concentration (270 micrograms/ml) reduced thraustochytrid growth by 50%, suggesting that natural concentrations are at least 15 times greater than that needed for significant microbiological effects. These results offer the first complete chemical characterization of a sea grass sulfated flavone glycoside and provide evidence that a secondary metabolite chemically defends T. testudinum against fouling microorganisms</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16349549</pmid><doi>10.1128/aem.64.4.1490-1496.1998</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Applied and environmental microbiology, 1998-04, Vol.64 (4), p.1490-1496 |
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| language | eng |
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| source | Open Access: PubMed Central; American Society for Microbiology Journals |
| subjects | ANGIOSPERMAS ANGIOSPERME ANGIOSPERMS Animal, plant and microbial ecology ANTIBIOTIC PROPERTIES ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Biological and medical sciences CHAMPIGNON DEFENCE MECHANISMS Fundamental and applied biological sciences. Psychology Fungal plant pathogens FUNGI General Microbial Ecology GLICOSIDOS GLYCOSIDE GLYCOSIDES Grasses HONGOS Marine MECANISME DE DEFENSE MECANISMOS DE DEFENSA Microbial ecology Microbiology Microflora of plants Pathology, epidemiology, host-fungus relationships. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection PROPIEDADES ANTIBIOTICAS PROPRIETE ANTIBIOTIQUE Thalassia testudinum |
| title | Evidence that a new antibiotic flavone glycoside chemically defends the sea grass Thalassia testudinum against zoosporic fungi |
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