Vacuolar acid hydrolysis as a physiological mechanism for sucrose breakdown

Sucrose breakdown in mature acidic 'Persian' limes (Citrus aurantifolia [Christm.] Swing.) occurred at a rate of 30.6 picomoles per milliliter per day during 9 weeks storage at 15 degrees C. Neither enzyme of sucrose catabolism (sucrose synthase or acid/alkaline invertase) was present in e...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1989-06, Vol.90 (2), p.530-533
Main Authors: Echeverria, E. (University of Florida, Lake Alfred, FL), Burns, J.K
Format: Article
Language:English
Subjects:
Acid hydrolysis
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Biological and medical sciences
Cell biochemistry
Cell physiology
CITRUS AURANTIFOLIA
Enzymes
FRUIT
FRUTO
Fundamental and applied biological sciences. Psychology
HIDROLISIS
HYDROLYSE
Juices
Metabolism and Enzymology
METABOLISME
METABOLISMO
Methylamines
Organic acids
Plant physiology and development
Plant tissues
PROTOPLASTE
PROTOPLASTO
Protoplasts
SUCROSA
SUCROSE
Sugars
Vacuoles
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Summary:Sucrose breakdown in mature acidic 'Persian' limes (Citrus aurantifolia [Christm.] Swing.) occurred at a rate of 30.6 picomoles per milliliter per day during 9 weeks storage at 15 degrees C. Neither enzyme of sucrose catabolism (sucrose synthase or acid/alkaline invertase) was present in extracts of mature storage tissue. The average vacuolar pH, estimated by direct measurement of sap from isolated vacuoles and by the methylamine method, was about 2.0 to 2.2. In vitro acid hydrolysis of sucrose at physiological concentrations in a buffered solution (pH 2.2) occurred at identical rates as in matured limes. The results indicate that sucrose breakdown in stored mature acidic limes occurs by acid hydrolysis
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.90.2.530