An analysis of the control of phosphorylation-coupled respiration in isolated plant mitochondria

The control of phosphorylation-coupled respiration in isolated turnip (Brassica rapa) mitochondria was investigated according to the principles of metabolic control analysis as developed by H. Kacser and J.A. Burns ([1973] Symp Soc Exp Biol 32: 65-104) and R. Heinrich and T.A. Rapoport ([1974] Eur J...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1989-07, Vol.90 (3), p.928-933
Main Authors: Anna C. Padovan, Dry, Ian B., Wiskich, Joseph T.
Format: Article
Language:English
Subjects:
ADENINA
ADENINE
Adenine nucleotides
ADENOSINDIFOSFATO
ADENOSINE DIPHOSPHATE
Biological and medical sciences
BRASSICA RAPA
Coefficients
Enzymes
FOSFORILACION
Fundamental and applied biological sciences. Psychology
HIDROLASAS
HYDROLASE
Liver mitochondria
Membranes and Bioenergetics
Metabolism
METALLOPROTEINE
METALPROTEINAS
Mitochondria
MITOCHONDRIE
MITOCONDRIA
NUCLEOTIDE
NUCLEOTIDOS
Oxidases
Oxidation
OXIDORREDUCTASAS
OXYDOREDUCTASE
PHOSPHORYLATION
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
RESPIRACION
RESPIRATION
Titration
Turnips
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Summary:The control of phosphorylation-coupled respiration in isolated turnip (Brassica rapa) mitochondria was investigated according to the principles of metabolic control analysis as developed by H. Kacser and J.A. Burns ([1973] Symp Soc Exp Biol 32: 65-104) and R. Heinrich and T.A. Rapoport ([1974] Eur J Biochem 42: 97-105). Inhibitor titration studies were used to determine quantitatively the amount of control exerted by four individual processes--cytochrome bc1, cytochrome oxidase, H+-ATPase, and the adenine nucleotide carrier--on respiratory flux under ADP-excess (state 3) and ADP-limited (state 4) conditions with a range of respiratory substrates. Under state 3 conditions control strength was found to be distributed between cytochrome oxidase, cytochrome bc1, and H+-ATPase in decreasing order of importance. The adenine nucleotide carrier exerted no control on respiratory flux under these conditions. Control strength at each step was found to vary with different substrates and with the respiratory flux as altered by ADP supply, i.e. virtually zero control strength at cytochrome oxidase and cytochrome bc1 under state 4 conditions
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.90.3.928