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Identification and characterization of the Ca2+ -ATPase which drives active transport of Ca2+ at the plasma membrane of radish seedlings

In microsomes from 24-hour-old radish (Raphanus sativus L.) seedlings ATP-dependent Ca2+ uptake occurs only in inside-out plasma membrane vesicles (F Rasi-Caldogno, MC Pugliarello, Ml De Michelis [1987] Plant Physiol 83:994-1000). A Ca2+ -dependent ATPase activity can be shown in the same microsomes...

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Published in:	Plant physiology (Bethesda) 1989-08, Vol.90 (4), p.1429-1434
Main Authors:	Rasi-Caldogno, F. (Universita di Torino, Torino, Italy), Pugliarello, M.C, Olivari, C, De Michelis, M.I
Format:	Article
Language:	English
Subjects:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Biological and medical sciences CALCIO CALCIUM ECHANGE D'ION Enzymes ESTRUCTURA CELULAR Fundamental and applied biological sciences. Psychology HIDROLASAS HYDROLASE INTERCAMBIO IONICO Membranes and Bioenergetics Metabolism Plant physiology and development PLANTULAS PLANTULE RAPHANUS SATIVUS STRUCTURE CELLULAIRE
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container_title	Plant physiology (Bethesda)
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creator	Rasi-Caldogno, F. (Universita di Torino, Torino, Italy) Pugliarello, M.C Olivari, C De Michelis, M.I
description	In microsomes from 24-hour-old radish (Raphanus sativus L.) seedlings ATP-dependent Ca2+ uptake occurs only in inside-out plasma membrane vesicles (F Rasi-Caldogno, MC Pugliarello, Ml De Michelis [1987] Plant Physiol 83:994-1000). A Ca2+ -dependent ATPase activity can be shown in the same microsomes, when assays are performed at pH 7.5. The Ca2+ -dependent ATPase is stimulated by the Ca2+ ionophore A23187 and is localized at the plasma membrane. Ca2+ -dependent ATPase activity and ATP-dependent Ca2+ uptake present very similar saturation kinetics with erythrosin B (50% inhibition at about 0.1 micromolar), free Ca2+ (half-maximal rate at about 70 nanomolar), and MgATP (Km 15-20 micromolar). Ca2+ uptake can be sustained by GTP or ITP at about 60% the rate measured in the presence of ATP; only very low Ca2+ uptake is sustained by CTP or UTP and none by ADP. These results indicate that the Ca2+ -ATPase described in this paper is the enzyme which drives active transport of Ca2+ at the plasma membrane of higher plants
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Psychology</topic><topic>HIDROLASAS</topic><topic>HYDROLASE</topic><topic>INTERCAMBIO IONICO</topic><topic>Membranes and Bioenergetics</topic><topic>Metabolism</topic><topic>Plant physiology and development</topic><topic>PLANTULAS</topic><topic>PLANTULE</topic><topic>RAPHANUS SATIVUS</topic><topic>STRUCTURE CELLULAIRE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rasi-Caldogno, F. (Universita di Torino, Torino, Italy)</creatorcontrib><creatorcontrib>Pugliarello, M.C</creatorcontrib><creatorcontrib>Olivari, C</creatorcontrib><creatorcontrib>De Michelis, M.I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rasi-Caldogno, F. 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The Ca2+ -dependent ATPase is stimulated by the Ca2+ ionophore A23187 and is localized at the plasma membrane. Ca2+ -dependent ATPase activity and ATP-dependent Ca2+ uptake present very similar saturation kinetics with erythrosin B (50% inhibition at about 0.1 micromolar), free Ca2+ (half-maximal rate at about 70 nanomolar), and MgATP (Km 15-20 micromolar). Ca2+ uptake can be sustained by GTP or ITP at about 60% the rate measured in the presence of ATP; only very low Ca2+ uptake is sustained by CTP or UTP and none by ADP. These results indicate that the Ca2+ -ATPase described in this paper is the enzyme which drives active transport of Ca2+ at the plasma membrane of higher plants</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16666947</pmid><doi>10.1104/pp.90.4.1429</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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source	JSTOR Archival Journals and Primary Sources Collection; Alma/SFX Local Collection
subjects	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Biological and medical sciences CALCIO CALCIUM ECHANGE D'ION Enzymes ESTRUCTURA CELULAR Fundamental and applied biological sciences. Psychology HIDROLASAS HYDROLASE INTERCAMBIO IONICO Membranes and Bioenergetics Metabolism Plant physiology and development PLANTULAS PLANTULE RAPHANUS SATIVUS STRUCTURE CELLULAIRE
title	Identification and characterization of the Ca2+ -ATPase which drives active transport of Ca2+ at the plasma membrane of radish seedlings
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