A two-dimensional vibrating probe study of currents around lateral roots of Raphanus sativus developing in culture

• Published in: Plant physiology (Bethesda) 1990-02, Vol.92 (2), p.543-546
• Main Authors: Rathore, K.S. (Purdue University, West Lafayette, IN), Hotary, K.B, Robinson, K.R
• Format: Article
• Language: English
• Subjects: ACIDE ACETIQUE; ACIDE INDOLACETIQUE; ACIDO ACETICO; ACIDO INDOLACETICO; APLICACIONES DEL ORDENADOR; APPLICATION DES ORDINATEURS; APPLICATION INFORMATIQUE; Biological and medical sciences; Cell growth; Cell physiology; Communications; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CULTURE DE TISSUS; Current density; Developmental biology; ECHANGE D'ION; Electric current; Electric fields; ELECTRICIDAD; ELECTRICITE; Electrodes; Fundamental and applied biological sciences. Psychology; INTERCAMBIO IONICO; MEDIO DE CULTIVO; Metabolism and Enzymology; MILIEU DE CULTURE; Plant physiology and development; Plant roots; Plants; PLANTULAS; PLANTULE; Plasma membrane and permeation; RACINE; RAICES; RAPHANUS SATIVUS; Root tips; Seedlings
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.92.2.543

A computer-assisted, two-dimensional vibrating probe was used to study the ionic currents around developing lateral roots of Raphanus sativus in vitro. This system allowed us the superimpose current vectors on the video image of the roots. In a young lateral root, current entered the cap, meristematic, and elongation zones and exited the primary root surface close to the base of the lateral root. As the lateral root grew, current began to exit from its basal (cell maturation zone) end. The densities of currents entering the apical portion of the faster-growing lateral roots in a medium lacking indole 3-acetic acid were about twice as large as those entering the apical region of the slower-growing lateral roots in indole 3-acetic acid-supplemented medium