104 Development of an Experimental Model to Induce Liver Abscesses in Steers Using an Acidotic Diet Challenge and Oral Bacterial Inoculation

• Published in: Journal of animal science 2023-11, Vol.101 (Supplement_3), p.68-69
• Main Authors: McDaniel, Zach S, Hales, Kristin E, Nagaraja, T G, Amachawadi, Raghavendra G, Lawrence, Ty E, Tennant, Travis C, Carroll, Jeff A, Burdick Sanchez, Nicole C, Galyean, Michael, Smock, Taylor M, Ballou, Michael A, Machado, Vinicius, Davis, Emily M, Broadway, Rand
• Format: Article
• Language: English
• Subjects: Abscesses; Bacteria; Culture techniques; Diet; Inoculation; Liver; Lungs; Mixtures; pH effects; Phylogeny; Polymerase chain reaction; Rumen
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skad281.082

Abstract The objective of this study was to develop a model for liver abscesses (LA) in Holstein steers by using an acidotic diet or acidotic diet plus intraruminal inoculation of Fusobacterium necrophorum, Salmonella enterica serotype Lubbock, and Truperella pyogenes. Holstein steers (n = 40; initial BW = 84.9 + 7.1 kg) were individually housed and randomly assigned to either a negative control diet (CON), an acidotic diet (AD), or an acidotic diet plus intraruminal inoculation with the bacterial mixture [ADB; (1 × 109 colony forming unit (CFU)/mL of each bacteria]. Steers in AD and ADB were cycled on (3 d) and off (2 d) a high-starch diet 20 days before intraruminal inoculation of ADB. Following inoculation, AD and ADB remained on the acidotic diet for the remainder of the study. Ruminal pH boluses were randomly inserted into 19 steers across treatments to monitor ruminal pH throughout the study. Steers were euthanized and necropsied to record gross pathology on the lung, rumen, liver, and colon. Culture techniques were used to determine the prevalence inoculated bacteria within LA. Quantitative polymerase chain reaction methods were used to quantify F. necrophorum in rumen tissue. Data were analyzed as a completely randomized design with animal as the experimental unit, where an α of ≤ 0.05 determined significance. Liver abscess prevalence was 43% in ADB vs. 0% in AD and CON treatments (P < 0.01). Ruminal damage was 51.1% greater in ADB than in AD and 78.6% greater than in CON (P ≤ 0.05). There were no pathological differences in the lungs and colons among treatment groups (P ≥ 0.14). There was a treatment × time interaction for ruminal pH, where pH decreased below 5.6 in AD and ADB calves during each cycle of the acidotic diet (P < 0.01). The ADB contained a greater concentration of F. necrophorum subsp. Necrophorum and subsp. Fundliforme within rumen tissue (P ≤ 0.03). Of the LA cultured, 100% contained F. necrophorum, 50% S. enterica, and 0% contained T. pyogenes. The F. necrophorum subsp. Necrophorum was phylogenetically typed and identical to the strain (FN-8LI) infused in the bacterial inoculation. These data suggest that acidotic diet in combination with intraruminal inoculation of the bacterial mixture is sufficient to induce liver abscesses and provides insight on possible mechanisms and origins of LA. Further studies are needed to validate these results and determine if this model may be used to study novel intervention methods.