PSVII-18 Applying the Marbling Window Concept to Enhance Intramuscular Fat Development in Beef Cattle

• Published in: Journal of animal science 2023-11, Vol.101 (Supplement_3), p.557-558
• Main Authors: Santos, Marta M, Costa, Thais C, Silva, Walmir, Pistillo, Leticia Z, Dean, Sarah E, Teixeira, Dante V, Sampaio, Claudia B, Gionbelli, Mateus P, Verardo, Lucas L, Paulino, Pedro Veiga Rodrigues, Duarte, Marcio S
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipogenesis; Age; Beef; Beef cattle; Body weight; Calves; Cattle; Deposition; Feed additives; Gene regulation; Immunofluorescence; Meat industry; Meat processing industry; Mesenchymal stem cells; miRNA; Mixtures; Neonates; Post-transcription; Preadipocytes; Progenitor cells; Proteins; Skeletal muscle; Stem cells; Weaning
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skad281.654

Abstract The increase in marbling deposition without increasing the overall fatness of the carcass is one of the most challenge goals in meat industry. Previous studies have suggested the nutritional interventions at the neonatal stages as a strategy to enhance the commitment of muscular mesenchymal stem cells to adipogenesis, which may contribute to enhance marbling deposition at the finishing phase. As such the objective of this study was designed the evaluate the impact nutrient supplementation during the pre-weaning stage of beef calves has on intramuscular adipogenic determination. Thirty-four female calves were assigned to two experimental treatments: Control (CON, n = 17), where calves received only a mineral mixture as a supplement; Supplemented (SUP, n = 17), where calves received an energy-protein supplement (0.5 kg·100 kg of BW-1·day-1; the same mineral mixture was used in the CON supplement). Calves were supplemented from 100 to 250 days of age. Once animals reached 250 days of age, skeletal muscle tissue samples were biopsied from the 10th and 11th rib and used for identification of mesenchymal stem cells and preadipocytes through immunofluorescence, mRNA and protein abundance of adipo-fibrogenic markers, and abundance of candidate miRNA controlling expression of adipogenic commitment. Data were analyzed considering the completely randomized design of the experiment. Heifers were randomized across paddocks, and paddocks were randomized across treatments. The PROC MIXED of SAS 9.2 was used for all analyses. The average daily gain (P = 0.08) and final body weight (P = 0.07) tended to be greater in SUP than CON calves. The number of fibro-adipogenic progenitor cells (P = 0.69) did not differ between treatments, while a greater number of intramuscular pre-adipocytes were observed in SUP than CON calves (P = 0.01). The expression of miRNA-4429 (P = 0.20) did not differ between treatments, while the expression of miRNA-129-5p (P = 0.09) and miRNA-129-2-3p (P = 0.05) tended to be greater in CON than SUP calves. Our findings demonstrate that energy-protein supplementation from 100 to 250 days of age is capable to enhance intramuscular adipogenesis in beef cattle. Moreover, our results suggest that regulation of adipogenic commitment occurs by post-transcriptional regulation through miR-129 family.