The effect of vitrification on blastocyst mitochondrial DNA dynamics and gene expression profiles

Purpose Does vitrification/warming affect the mitochondrial DNA (mtDNA) content and the gene expression profile of blastocysts? Methods Prospective cohort study in which 89 blastocysts were obtained from 50 patients between July 2017 and August 2018. mtDNA was measured in a total of 71 aneuploid bla...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2023-11, Vol.40 (11), p.2577-2589
Main Authors: Pérez-Sánchez, Marta, Pardiñas, Maria Luisa, Díez-Juan, Antonio, Quiñonero, Alicia, Domínguez, Francisco, Martin, Angel, Vidal, Carmina, Beltrán, Diana, Mifsud, Amparo, Mercader, Amparo, Pellicer, Antonio, Cobo, Ana, de los Santos, Maria José
Format: Article
Language:English
Subjects:
Aneuploidy
Apoptosis
Blastocyst - physiology
Blastocysts
Cell culture
Cellular stress response
Cryopreservation - methods
DNA damage
DNA, Mitochondrial - genetics
Embryo Biology
Embryo Culture Techniques
Gene expression
Gene set enrichment analysis
Gynecology
Human Genetics
Humans
Immune response (humoral)
Medicine
Medicine & Public Health
Mitochondrial DNA
Polymerase chain reaction
Prospective Studies
Reproductive Medicine
Transcriptome - genetics
Transcriptomics
Vitrification
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Summary:Purpose Does vitrification/warming affect the mitochondrial DNA (mtDNA) content and the gene expression profile of blastocysts? Methods Prospective cohort study in which 89 blastocysts were obtained from 50 patients between July 2017 and August 2018. mtDNA was measured in a total of 71 aneuploid blastocysts by means of real-time polymerase chain reaction (RT-PCR). Transcriptomic analysis was performed by RNA sequencing (RNA-seq) in an additional 8 aneuploid blastocysts cultured for 0 h after warming, and 10 aneuploid blastocysts cultured for 4–5 h after warming. Results A significant decrease in mtDNA content just during the first hour after the warming process in blastocysts was found ( P  
ISSN:1058-0468
1573-7330
1573-7330
DOI:10.1007/s10815-023-02952-3