Variant cell lines of Haplopappus gracilis with disturbed activities of nitrate reductase and nitrite reductase

• Published in: Plant physiology (Bethesda) 1985-07, Vol.78 (3), p.658-660
• Main Authors: Gilissen, L.J.W, Barneix, A.J, Staveren, M.V, Breteler, H
• Format: Article
• Language: English
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Agronomy. Soil science and plant productions; Biological and medical sciences; Cell culture techniques; Cell cultures. Hybridization. Fusion; Cell growth; Cell lines; CELLS; CELLULE; CELULAS; Chromosomes; Cultured cells; Economic plant physiology; Enzymes; ENZYMIC ACTIVITY; Fundamental and applied biological sciences. Psychology; HAPLOPAPPUS; Molecular and cellular biology; Nitrates; NITRITE REDUCTASE; Nitrites; Nitrogen metabolism and other ones (excepting carbon metabolism); Nutrition. Photosynthesis. Respiration. Metabolism; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plant cells; Plant cells and fungal cells; Plants; Short Communications
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.78.3.658

Selected variant cell lines of Haplopappus gracilis (Nutt) Gray that showed disturbed growth after transfer from an alanine medium to NO3 - medium were characterized. The in vivo NO3 - reductase activity (NRA) was lower in these lines than in the wild type. In vitro NRA assays suggest that decreased in vivo NRA was not caused by a lower amount of active enzyme. Cells of the variant lines revealed up to 75% lower extractable activity of $\text{NO}_{2}{}^{-}$ reductase as compared with the wild type. This coincided with higher accumulation of $\text{NO}_{2}{}^{-}$ by the variant than by the wild type cells after transfer from alanine medium to NO3 - medium. $\text{NO}_{2}{}^{-}$ accumulation was transient or continuous, depending on cell line, metabolic state of the cells, and light conditions.