Inhibition of oxygen evolution in chloroplasts by ferricyanide [Pisum sativum, pea leaves]

Preincubation of chloroplasts from pea leaves (Pisum sativum L. cv. Kelvedon) with 0.5 millimolar ferricyanide in the dark, caused a parallel inhibition of the rate of rise of the variable fluorescence and the rate of electron transport. Both reactions were inhibited to a similar extent by varying t...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1982-09, Vol.70 (3), p.840-843
Main Authors: Drechsler, Z, Neumann, J
Format: Article
Language:English
Subjects:
Anions
Chloroplasts
Fluorescence
Hydroquinones
Kinetics
Luminous intensity
Oxidation
Oxygen
Photosystem II
Sieving
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Summary:Preincubation of chloroplasts from pea leaves (Pisum sativum L. cv. Kelvedon) with 0.5 millimolar ferricyanide in the dark, caused a parallel inhibition of the rate of rise of the variable fluorescence and the rate of electron transport. Both reactions were inhibited to a similar extent by varying the time of preincubation, the concentration of ferricyanide during preincubation, and by raising the concentration of salts in the preincubation medium. Ferricyanide treatment of Tris-washed chloroplasts did not inhibit electron transport from the Photosystem II (PSII) electron donor 1,5-diphenylcarbazide to methylviologen. The inhibition of the variable fluorescence rise and of NADP reduction (caused by ferricyanide pretreatment) was bypassed by addition of the PSII electron donor couple hydroquinone/ascorbate. It was concluded that preincubation of chloroplasts with ferricyanide in the dark inhibited electron transport between water and PSII.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.70.3.840