High Rates of Protein Synthesis by Isolated Chloroplasts

Improvements are described in the preparation and in vitro conditions of an intact pea (Pisum sativum Progress No. 9) chloroplast system which provides high efficiency for translation of endogenous messenger RNA, using light as an energy source. High rates result in the incorporation into protein of...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1982-10, Vol.70 (4), p.1107-1114
Main Authors: Leonard E. Fish, Jagendorf, Andre T.
Format: Article
Language:English
Subjects:
Amino acids
Chloroplasts
Gels
Messenger RNA
Peas
Plants
Protein synthesis
Ribosomes
Spinach
Thylakoids
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Summary:Improvements are described in the preparation and in vitro conditions of an intact pea (Pisum sativum Progress No. 9) chloroplast system which provides high efficiency for translation of endogenous messenger RNA, using light as an energy source. High rates result in the incorporation into protein of up to 100 nanomoles tritiated leucine per milligram chlorophyll. These rates suggest extensive reinitiation, and repeated utilization of the messenger RNA that code for thylakoid proteins. Up to 39 radioactive thylakoid peptide bands were detected by fluorography after labeling with tritiated leucine.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.70.4.1107