Dimerization mechanism of an inverted-topology ion channel in membranes

• Published in: Proceedings of the National Academy of Sciences - PNAS 2023-11, Vol.120 (47), p.e2308454120-e2308454120
• Main Authors: Ernst, Melanie, Orabi, Esam A, Stockbridge, Randy B, Faraldo-Gómez, José D, Robertson, Janice L
• Format: Article
• Language: English
• Subjects: Assembly; Binding Sites; Biological Sciences; Defects; Dimerization; Dimers; Fluorides; Interface stability; Ion channels; Ion Channels - metabolism; Lipid bilayers; Lipid Bilayers - chemistry; Lipids; Membranes; Molecular dynamics; Oligomerization; Physical Sciences; Stable channels; Topology
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2308454120

Many ion channels are multisubunit complexes where oligomerization is an obligatory requirement for function as the binding axis forms the charged permeation pathway. However, the mechanisms of in-membrane assembly of thermodynamically stable channels are largely unknown. Here, we demonstrate a key advance by reporting the dimerization equilibrium reaction of an inverted-topology, homodimeric fluoride channel Fluc in lipid bilayers. While the wild-type channel is a long-lived dimer, we leverage a known mutation, N43S, that weakens Na binding in a buried site at the interface, thereby unlocking the complex for reversible association in lipid bilayers. Single-channel recordings show that Na binding is required for fluoride conduction while single-molecule microscopy experiments demonstrate that N43S Fluc exists in a dynamic monomer-dimer equilibrium in the membrane, even following removal of Na . Quantifying the thermodynamic stability while titrating Na indicates that dimerization occurs first, providing a membrane-embedded binding site where Na binding weakly stabilizes the complex. To understand how these subunits form stable assemblies while presenting charged surfaces to the membrane, we carried out molecular dynamics simulations, which show the formation of a thinned membrane defect around the exposed dimerization interface. In simulations where subunits are permitted to encounter each other while preventing protein contacts, we observe spontaneous and selective association at the native interface, where stability is achieved by mitigation of the membrane defect. These results suggest a model wherein membrane-associated forces drive channel assembly in the native orientation while subsequent factors, such as Na binding, result in channel activation.