Draft genome sequencing of halotolerant bacterium Salinicola sp. DM10 unravels plant growth-promoting potentials

• Published in: 3 Biotech 2023-12, Vol.13 (12), p.416, Article 416
• Main Authors: Nguyen, Ngoc-Lan, Van Dung, Vu, Van Tung, Nguyen, Nguyen, Thi Kim Lien, Quan, Nguyen Duc, Giang, Tran Thi Huong, Ngan, Nguyen Thi Thanh, Hien, Nguyen Thanh, Nguyen, Huy-Hoang
• Format: Article
• Language: English
• Subjects: 1-Aminocyclopropane-1-carboxylate deaminase; Agriculture; Alkaline phosphatase; Amy1A gene; Bacteria; Bioinformatics; Biomaterials; Biotechnology; Cancer Research; Carboxylic acids; Chemistry; Chemistry and Materials Science; Gene sequencing; Genes; Genome Reports; Genomes; Gluconic acid; Iron; Plant growth; Salinity tolerance; Sodium chloride; Solubilization; Stem Cells; Transportation systems; α-Amylase
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-023-03833-3

In this study, strain DM10 was isolated from mangrove roots and characterized as a halotolerant plant growth-promoting bacterium. Strain DM10 exhibited the ability to solubilize phosphate, produce siderophore, show 1-aminocyclopropane-1-carboxylic acid deaminase activity, and hydrolyze starch. The rice plants subjected to a treatment of NaCl (200 mM) and inoculated with strain DM10 showed an improvement in the shoot length, root length, and dried weight, when compared to those exposed solely to saline treatment. The comprehensive genome sequencing of strain DM10 revealed a genome spanning of 4,171,745 bp, harboring 3626 protein coding sequences. Within its genome, strain DM10 possesses genes responsible for both salt-in and salt-out strategies, indicative of a robust genetic adaptation aimed at fostering salt tolerance. Additionally, the genome encodes genes involved in phosphate solubilization, such as the synthesis of gluconic acid, high-affinity phosphate transport systems, and alkaline phosphatase. In the genome of DM10, we identified the acdS gene, responsible for encoding 1-aminocyclopropane-1-carboxylate deaminase, as well as the amy1A gene, which encodes α-amylase. Furthermore, the genome of DM10 contains sequences associated with the iron (3 + )-hydroxamate and iron uptake clusters, responsible for siderophore production. Such data provide a deep understanding of the mechanism employed by strain DM10 to combat osmotic and salinity stress, facilitate plant growth, and elucidate its molecular-level behaviors.