Heat Shock Proteins in Tobacco Cell Suspension during Growth Cycle 1

• Published in: Plant physiology (Bethesda) 1984-07, Vol.75 (3), p.639-644
• Main Authors: Kanabus, Jan, Pikaard, Craig S., Cherry, Joe H.
• Format: Article
• Language: English
• ISSN: 0032-0889; 1532-2548

Tobacco ( Nicotiana tabacum L. cv Wisconsin 38) cells grown in suspension culture at 26°C produce heat shock proteins (HSPs) when exposed to elevated temperature of 34 to 42°C. At 34 and 38°C, synthesis of normal proteins is maintained while HSPs are expressed within 30 minutes after initiation of the shock. At 42°C, HSPs are still expressed but normal proteins are made at a reduced rate or not at all. Exposure of cells to 38°C allows for a full expression of HSPs without inhibition of the synthesis of normal proteins. Induced synthesis of HSPs at 38°C is maximal 1 to 2 hours after elevation of temperature and diminishes thereafter through at least 6 hours. Cells growing asynchronously in the logarithmic phase of growth produce HSPs at a much higher rate than those in the stationary phase. The ability to synthesize HSPs disappears about one generation time before the cells reach a growth plateau.