Suberin production by isolated tomato fruit protoplasts [Multilamellar walls]

The multilamellar wall secreted by protoplasts isolated from locule tissue of tomato (Lycopersicon esculentum L.) fruit was purified, and an extract was obtained after depolymerization with $\text{BF}_{3}$-methanol. Analysis of this extract using thin layer chromatography demonstrated the presence o...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1984-07, Vol.75 (3), p.716-719
Main Authors: RAO, G. S. R. L, MARTIN WILLISON, J. H, NIMAL RATNAYAKE, W. M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Dicarboxylic acids
Esters
Fatty acids
Fatty alcohols
Fundamental and applied biological sciences. Psychology
Lipids
Materials
Plant physiology and development
Plant tissues
Plants
Protoplast culture
Protoplasts
Tissue cultures, protoplasts
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Summary:The multilamellar wall secreted by protoplasts isolated from locule tissue of tomato (Lycopersicon esculentum L.) fruit was purified, and an extract was obtained after depolymerization with $\text{BF}_{3}$-methanol. Analysis of this extract using thin layer chromatography demonstrated the presence of fatty acid methyl esters, fatty alcohols, dicarboxylic acid dimethyl esters, and ω-hydroxy acid methyl esters. These components were quantified using an latroscan thin layer chromatography-flame ionization detection system. The different chain lengths in each group were identified and quantified using gas chromatography. The results clearly indicated the presence of suberin.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.75.3.716