Evidence of singlet oxygen participation in the chlorophyll-sensitized photooxidation of indoleacetic acid [Peas, Pisum sativum]

Chlorophyll-sensitized photooxidation of indoleacetic acid (IAA)--with chlorophyll extracted from Pisum sativum L. cv. Alaska W.R.--was determined in the presence of deuterium oxide and known quenchers of singlet oxygen (^{1}\text{O}{}_{2}$) to explore the involvement of ^{1}\text{O}{}_{2}$ in the r...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1982-08, Vol.70 (2), p.414-417
Main Authors: Koch, J.L, Oberlander, R.M, Tamas, I.A, Germain, J.L, Ammondson, D.B.S
Format: Article
Language:English
Subjects:
Azides
Hydroquinones
Hydroxyl radicals
Insulin antibodies
Oxidases
Oxidation
Photooxidation
Singlet oxygen
Superoxides
Xanthines
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Summary:Chlorophyll-sensitized photooxidation of indoleacetic acid (IAA)--with chlorophyll extracted from Pisum sativum L. cv. Alaska W.R.--was determined in the presence of deuterium oxide and known quenchers of singlet oxygen (^{1}\text{O}{}_{2}$) to explore the involvement of ^{1}\text{O}{}_{2}$ in the reaction. O2 uptake was measured in light in a buffered aqueous micellar system containing Triton X-100, KCl, chlorophyll, and IAA. The rate of O2 uptake was zero in darkness. The reaction was stimulated by deuterium oxide and inhibited by sodium azide indicating that ^{1}\text{O}{}_{2}$ participated in IAA photooxidation. Both mannitol and superoxide dismutase failed to inhibit O2 uptake suggesting that neither the hydroxyl radical nor the superoxide anion played a significant role in the reaction.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.70.2.414