Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin [Cottonwood, Populus deltoides]

It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique fo...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1982-08, Vol.70 (2), p.606-609
Main Authors: Vogelmann, Thomas C., Dickson, Richard E.
Format: Article
Language:English
Subjects:
Cell walls
Epoxy resins
Freeze drying
Freezing
Liquids
Petioles
Phloem
Plant tissues
Resins
Specimens
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Summary:It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique for the preparation of microautoradiographs of 14C-labeled, water-soluble compounds in plant tissue. Samples from cottonwood (Populus deltoides Bartr. ex Marsh.) labeled with 14C were excised, quick frozen in liquid N2, freeze-dried at -50°C, and pressure-infiltrated with epoxy resin without intermediate solvents or prolonged incubation times. The technique facilitates the mass processing of samples for microautoradiography, gives good cellular retention of labeled water-soluble compounds, and is highly reproducible.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.70.2.606