Effects of blister blight disease on endophytic microbial diversity and community structure in tea (Camellia sinensis) leaves

• Published in: 3 Biotech 2023-12, Vol.13 (12), p.421, Article 421
• Main Authors: Cao, Rui, Dong, Xuan, Zhao, Yichen, Yin, Jie
• Format: Article
• Language: English
• Subjects: Abundance; Actinobacteria; Agriculture; Bacteria; Bioinformatics; Biomaterials; Biotechnology; Blight; Blistering; Cancer Research; Chemistry; Chemistry and Materials Science; Community structure; Comparative analysis; Disease; Endophytes; Leaves; Metagenomics; Microorganisms; Original; Original Article; Stem Cells; Tea
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-023-03846-y

In this study, metagenomic sequencing technology was employed to analyze the ITS1 region sequence of the ITS rDNA gene of endophytic fungi and 16S sequence of endophytic bacteria in tea leaves with varying degrees of infection by tea blister blight disease as well as healthy tea leaves. Subsequently, a comparative analysis was conducted on the endophytic microbial diversity and the community structure in tea leaves. The findings of this investigation reveal a shift in the dominant endophytic fungal genera from Ascomycota to Basidiomycota as the disease progressed. Furthermore, a negative correlation was observed between Exobasidium and Talaromyce , with Talaromyce exhibiting potential as an antagonist against the disease. Meanwhile, our findings reveal that Proteobacteria , Firmicutes , and Actinobacteria were the three most abundant bacteria phyla in tea leaves. As the disease progressed, there was an increase in the relative abundance of Actinobacteria , while Variovorax , Sphingomonas , and Pseudomonas were found to have higher abundance in later stages. The diversity analysis results indicated that the endophytic microbial diversity and the community structure in tea leaves in the diseased group were lower than those in the healthy control group. In general, blister blight disease altered the community structure of endophytic microorganisms in tea leaves, resulting in a few species with high abundance. The study lays a foundation for investigating the pathogenic mechanism of tea blister disease and establishing a theoretical basis for controlling diseases in tea trees.