Exoribonuclease RNase R protects Antarctic Pseudomonas syringae Lz4W from DNA damage and oxidative stress

Bacterial exoribonucleases play a crucial role in RNA maturation, degradation, quality control, and turnover. In this study, we have uncovered a previously unknown role of 3'-5' exoribonuclease RNase R of Lz4W in DNA damage and oxidative stress response. Here, we show that neither the exor...

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Published in:Applied and environmental microbiology 2023-11, Vol.89 (11), p.e0116823-e0116823
Main Authors: Mittal, Pragya, Sipani, Rashmi, Pandiyan, Apuratha, Sulthana, Shaheen, Sinha, Anurag K, Hussain, Ashaq, Ray, Malay K, Pavankumar, Theetha L
Format: Article
Language:English
Subjects:
Antarctic Regions
Bacteria
Biodegradation
Cold shock proteins
Damage
Degradation
Deletion mutant
Deoxyribonucleic acid
DNA
DNA Damage
Environmental Microbiology
Escherichia coli - genetics
Escherichia coli - metabolism
Exoribonucleases - genetics
Exoribonucleases - metabolism
Gene deletion
Low temperature
Nucleic acids
Oxidative Stress
Pseudomonas
Pseudomonas syringae
Pseudomonas syringae - genetics
Pseudomonas syringae - metabolism
Ribonuclease E
Ribonucleic acid
RNA
RNA processing
RNA, Bacterial - genetics
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Summary:Bacterial exoribonucleases play a crucial role in RNA maturation, degradation, quality control, and turnover. In this study, we have uncovered a previously unknown role of 3'-5' exoribonuclease RNase R of Lz4W in DNA damage and oxidative stress response. Here, we show that neither the exoribonuclease function of RNase R nor its association with the RNA degradosome complex is essential for this function. Interestingly, in Lz4W, hydrolytic RNase R exhibits physiological roles similar to phosphorolytic 3'-5' exoribonuclease PNPase of . Our data suggest that during the course of evolution, mesophilic and psychrotrophic have apparently swapped these exoribonucleases to adapt to their respective environmental growth conditions.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.01168-23