Global Urinary Volatolomics with (GC×)GC-TOF-MS

Urinary volatolomics offers a noninvasive approach for disease detection and monitoring. Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-11, Vol.95 (47), p.17170-17176
Main Authors: Myridakis, Antonis, Wen, Qing, Boshier, Piers R, Parker, Aaron G, Belluomo, Ilaria, Handakas, Evangelos, Hanna, George B
Format: Article
Language:English
Subjects:
Adenocarcinoma
Biomarkers
Chromatography, Gas
Column chromatography
Data analysis
Disease detection
Gas chromatography
Gas Chromatography-Mass Spectrometry - methods
Humans
Organic compounds
Sorbents
Technical Note
VOCs
Volatile organic compounds
Volatile Organic Compounds - analysis
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Summary:Urinary volatolomics offers a noninvasive approach for disease detection and monitoring. Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column gas chromatography (GC) assay yielded substantially higher numbers of monitored VOCs compared to our previously reported single-sorbent method. Multidimensional GC (GC×GC) enhanced further biomarker discovery while data analysis was simplified by using a tile-based approach. At the same time, the required urine volume was reduced 5-fold from 2 to 0.4 mL. The applicability of the methodology was demonstrated in a pancreatic ductal adenocarcinoma cohort where previous findings were confirmed while a series of additional VOCs with diagnostic potential were discovered.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c02523