Phage Milagro: a platform for engineering a broad host range virulent phage for Burkholderia

infections are a significant concern in people with CF and other immunocompromising disorders, and are difficult to treat with conventional antibiotics due to their inherent drug resistance. Bacteriophages, or bacterial viruses, are now seen as a potential alternative therapy for these infections, b...

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Bibliographic Details
Published in:Journal of virology 2023-11, Vol.97 (11), p.e0085023
Main Authors: Yao, Guichun, Le, Tram, Korn, Abby M, Peterson, Hannah N, Liu, Mei, Gonzalez, Carlos F, Gill, Jason J
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Bacteriophages - genetics
Burkholderia - virology
Burkholderia Infections - therapy
Cystic Fibrosis - microbiology
Genetic Diversity and Evolution
Host Specificity
Humans
Phage Therapy
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Summary:infections are a significant concern in people with CF and other immunocompromising disorders, and are difficult to treat with conventional antibiotics due to their inherent drug resistance. Bacteriophages, or bacterial viruses, are now seen as a potential alternative therapy for these infections, but most of the naturally occurring phages are temperate and have narrow host ranges, which limit their utility as therapeutics. Here we describe the temperate phage Milagro and our efforts to engineer this phage into a potential therapeutic by expanding the phage host range and selecting for phage mutants that are strictly virulent. This approach may be used to generate new therapeutic agents for treating intractable infections in CF patients.
ISSN:0022-538X
1098-5514
1098-5514
DOI:10.1128/jvi.00850-23