Reorganization of lamina-associated domains in early mouse embryos is regulated by RNA polymerase II activity

Fertilization in mammals is accompanied by an intense period of chromatin remodeling and major changes in nuclear organization. How the earliest events in embryogenesis, including zygotic genome activation (ZGA) during maternal-to-zygotic transition, influence such remodeling remains unknown. Here,...

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Bibliographic Details
Published in:Genes & development 2023-10, Vol.37 (19-20), p.901-912
Main Authors: Pal, Mrinmoy, Altamirano-Pacheco, Luis, Schauer, Tamas, Torres-Padilla, Maria-Elena
Format: Article
Language:English
Subjects:
Animals
Chromatin
Embryonic Development - genetics
Gene Expression Regulation, Developmental
Mammals - genetics
Mice
Research Papers
RNA Polymerase II - genetics
Transcription, Genetic
Zygote
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Summary:Fertilization in mammals is accompanied by an intense period of chromatin remodeling and major changes in nuclear organization. How the earliest events in embryogenesis, including zygotic genome activation (ZGA) during maternal-to-zygotic transition, influence such remodeling remains unknown. Here, we have investigated the establishment of nuclear architecture, focusing on the remodeling of lamina-associated domains (LADs) during this transition. We report that LADs reorganize gradually in two-cell embryos and that blocking ZGA leads to major changes in nuclear organization, including altered chromatin and genomic features of LADs and redistribution of H3K4me3 toward the nuclear lamina. Our data indicate that the rearrangement of LADs is an integral component of the maternal-to-zygotic transition and that transcription contributes to shaping nuclear organization at the beginning of mammalian development.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.350799.123