Mismatch repair in Escherichia coli cells lacking single-strand exonucleases ExoI, ExoVII, and RecJ

In vitro, the methyl-directed mismatch repair system of Escherichia coli requires the single-strand exonuclease activity of either ExoI, ExoVII, or RecJ and possibly a fourth, unknown single-strand exonuclease. We have created the first precise null mutations in genes encoding ExoI and ExoVII and fi...

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Bibliographic Details
Published in:Journal of bacteriology 1998-02, Vol.180 (4), p.989-993
Main Authors: Harris, R S, Ross, K J, Lombardo, M J, Rosenberg, S M
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriology
Biochemistry
DNA Repair
DNA, Single-Stranded - metabolism
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Proteins
Exodeoxyribonucleases - deficiency
Exodeoxyribonucleases - genetics
Genetics and Molecular Biology
Mutagenesis
Mutation
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Summary:In vitro, the methyl-directed mismatch repair system of Escherichia coli requires the single-strand exonuclease activity of either ExoI, ExoVII, or RecJ and possibly a fourth, unknown single-strand exonuclease. We have created the first precise null mutations in genes encoding ExoI and ExoVII and find that cells lacking these nucleases and RecJ perform mismatch repair in vivo normally such that triple-null mutants display normal mutation rates. ExoI, ExoVII, and RecJ are either redundant with another function(s) or are unnecessary for mismatch repair in vivo.
ISSN:0021-9193
1098-5530
DOI:10.1128/JB.180.4.989-993.1998