Widespread Distribution of the arsO Gene Confers Bacterial Resistance to Environmental Antimony

• Published in: Environmental science & technology 2023-10, Vol.57 (39), p.14579-14588
• Main Authors: Tang, Shi-Tong, Song, Xin-Wei, Chen, Jian, Shen, Jie, Ma, Bin, Rosen, Barry P., Zhang, Jun, Zhao, Fang-Jie
• Format: Article
• Language: English
• Subjects: Adenine; Antimony; Antimony - chemistry; Antimony - metabolism; Arsenic; Arsenite; Biogeochemical Cycling; Detoxification; E coli; Enzymatic activity; Escherichia coli - metabolism; Flavin; Flavin-adenine dinucleotide; Flavin-Adenine Dinucleotide - metabolism; Genomic analysis; Humans; Interleukin-1 Receptor-Like 1 Protein - metabolism; Microorganisms; Nicotinamide adenine dinucleotide; Oxidation; Oxidation resistance; Oxidation-Reduction; Oxidoreductases - metabolism; Soil contamination; Soil pollution; Stibnite
• ISSN: 0013-936X; 1520-5851; 1520-5851
• DOI: 10.1021/acs.est.3c03458

Microbial oxidation of environmental antimonite (Sb­(III)) to antimonate (Sb­(V)) is an antimony (Sb) detoxification mechanism. Ensifer adhaerens ST2, a bacterial isolate from a Sb-contaminated paddy soil, oxidizes Sb­(III) to Sb­(V) under oxic conditions by an unknown mechanism. Genomic analysis of ST2 reveals a gene of unknown function in an arsenic resistance (ars) operon that we term arsO. The transcription level of arsO was significantly upregulated by the addition of Sb­(III). ArsO is predicted to be a flavoprotein monooxygenase but shows low sequence similarity to other flavoprotein monooxygenases. Expression of arsO in the arsenic-hypersensitive Escherichia coli strain AW3110Δars conferred increased resistance to Sb­(III) but not arsenite (As­(III)) or methylarsenite (MAs­(III)). Purified ArsO catalyzes Sb­(III) oxidation to Sb­(V) with NADPH or NADH as the electron donor but does not oxidize As­(III) or MAs­(III). The purified enzyme contains flavin adenine dinucleotide (FAD) at a ratio of 0.62 mol of FAD/mol protein, and enzymatic activity was increased by addition of FAD. Bioinformatic analyses show that arsO genes are widely distributed in metagenomes from different environments and are particularly abundant in environments affected by human activities. This study demonstrates that ArsO is an environmental Sb­(III) oxidase that plays a significant role in the detoxification of Sb­(III).