Effect of different concentrations of titanium and silver nanoparticles on maxillofacial silicone MDX4-4210 on cell viability, tear bond strength, and shore strength: An in vitro study

• Published in: The journal of Indian Prosthodontic Society 2023-10, Vol.23 (4), p.384-390
• Main Authors: Kumari, Shweta, John, Peter, Chidambaranathan, Ahila, Muthukumar, Balasubramanium
• Format: Article
• Language: English
• Subjects: Bonds; Cell lines; Cell Survival; Cell viability; Hardness; Materials Testing; Maxillofacial; Metal Nanoparticles; Nanoparticles; Silicone Elastomers - chemistry; Silicone Elastomers - pharmacology; Silicones; Silver; Silver - pharmacology; Statistical analysis; Tensile Strength; Titanium; Titanium - pharmacology; Titanium dioxide
• ISSN: 0972-4052; 1998-4057; 1998-4057
• DOI: 10.4103/jips.jips_378_23

Aim: The aim was to evaluate and compare the cell viability, tear bond strength, and shore hardness of MDX4-4210 silicone reinforced with titanium dioxide and silver nanoparticles in 1%, 2%, and 3%. Settings and Design: The study design involves in vitro comparative study. Materials and Methods: MDX4-4210 silicone incorporated with 1%, 2%, and 3% by weight of silver and titanium dioxide nanoparticles. A total of 112 specimens were prepared and grouped into 7, with 16 specimens in each group. Group 1 - control, Groups 2, 3, and 4 were 1%, 2%, and 3% silver, respectively. Groups 5, 6, and 7 were 1%, 2%, and 3% titanium dioxide nanoparticles, respectively. Cell viability was tested by MTT ASSAY on MG63 cell lines, tear bond strength was tested by peeling force in universal testing machine, and Shore A hardness was tested in durometer. Statistical Analysis Used: The cell viability values were statistically analysed using one-way analysis of variance, and Tukey honestly significant difference test, tear bond strength and shore hardness values were analysed using Mann-Whitney test. Results: Based on the MTT ASSAY test, 1% silver nanoparticles incorporated MDX4-4210 silicone showed maximum cell viability of 42.10%, whereas minimum cell viability was 18.06% for 3% of titanium dioxide-reinforced silicone. The mean value of tear bond strength of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 62.81 ± 3.637 N/m and 59.69 ± 5.313 N/m and the mean value of shore hardness of room temperature vulcanized of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 38.06 ± 1.237 and 36.75 ± 1.291. Conclusion: Cell viability of 1% silver nanoparticles reinforced MDX4-4210 silicone was higher in comparison to the other groups, and tear bond strength and shore hardness were significantly higher in 1% silver nanoparticles reinforced silicone compared to 1% titanium nanoparticles reinforced MDX4-4210 silicone.