Live-cell visualization of the trans-cellular mode of monocyte transmigration across the vascular endothelium, and its relationship with endothelial PECAM-1

In response to atherogenic stimuli, blood monocytes transmigrate across the vascular endothelium not only through endothelial cell-cell junctions (para-cellular) but also through endothelial cells themselves (trans-cellular). The molecular mechanism of the latter is mostly unknown, because it rarely...

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Bibliographic Details
Published in:The journal of physiological sciences 2012-01, Vol.62 (1), p.63-69
Main Authors: Hashimoto, Ken, Kataoka, Noriyuki, Nakamura, Emi, Hagihara, Kimiko, Okamoto, Takeaki, Kanouchi, Hiroaki, Mohri, Satoshi, Tsujioka, Katsuhiko, Kajiya, Fumihiko
Format: Article
Language:English
Subjects:
Cell Line
Cell Movement - physiology
Endothelial Cells - cytology
Endothelium, Vascular - cytology
Humans
Intercellular Junctions
Monocytes - physiology
Platelet Endothelial Cell Adhesion Molecule-1 - physiology
Short Communication
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Summary:In response to atherogenic stimuli, blood monocytes transmigrate across the vascular endothelium not only through endothelial cell-cell junctions (para-cellular) but also through endothelial cells themselves (trans-cellular). The molecular mechanism of the latter is mostly unknown, because it rarely happens, especially in vitro. Although many reports have recognized trans-cellular migration from snapshot images of leukocytes halfway across the endothelium at non-junctional locations, it often produces a false-positive result, because some leukocytes that initiate trans-cellular migration withdraw and return to the apical endothelial surface. Thus, analyzing the entire process is essential. In this study, complete monocyte trans-cellular migration was successfully captured for live cells, with simultaneous visualization of endothelial PECAM-1. We suggest the possible existence of both PECAM-1-related migration at peri-junctional sites and PECAM-1-unrelated migration at sites remote from junctions. This is the first report to describe the entire process of monocyte trans-cellular migration for live cells and its relationship with endothelial PECAM-1.
ISSN:1880-6546
1880-6562
DOI:10.1007/s12576-011-0181-8