Neuroprotective effects of oleuropein against cognitive dysfunction induced by colchicine in hippocampal CA1 area in rats

• Published in: The journal of physiological sciences 2016-09, Vol.66 (5), p.397-405
• Main Authors: Pourkhodadad, Soheila, Alirezaei, Masoud, Moghaddasi, Mehrnoush, Ahmadvand, Hassan, Karami, Manizheh, Delfan, Bahram, Khanipour, Zahra
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Animals; Antioxidants; Behavior, Animal - drug effects; CA1 Region, Hippocampal - drug effects; CA1 Region, Hippocampal - metabolism; Caspase 3 - metabolism; Catalase - metabolism; Cognitive Dysfunction - chemically induced; Cognitive Dysfunction - drug therapy; Cognitive Dysfunction - metabolism; Colchicine; Ethylenediaminetetraacetic acid; Glutathione Peroxidase - metabolism; Iridoid Glucosides; Iridoids - pharmacology; Iridoids - therapeutic use; Lipid Peroxidation - drug effects; Male; Malondialdehyde - metabolism; Medical research; Medicine, Experimental; Nervous system diseases; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Nitric oxide; Nitric Oxide - metabolism; Original Paper; Oxidative Stress - drug effects; Rats; Rats, Wistar; Spatial Learning - drug effects; Spatial Memory - drug effects
• ISSN: 1880-6546; 1880-6562
• DOI: 10.1007/s12576-016-0437-4

Alzheimer's disease is a progressive neurodegenerative disorder with decline in memory. The role of oxidative stress is well known in the pathogenesis of the disease. The purpose of this study was to evaluate pretreatment effects of oleuropein on oxidative status and cognitive dysfunction induced by colchicine in the hippocampal CA1 area. Male Wistar rats were pretreated orally once daily for 10 days with oleuropein at doses of 10, 15 and 20 mg/kg. Thereafter, colchicine (15 μg/rat) was administered into the CA1 area of the hippocampus to induce cognitive dysfunction. The Morris water maze was used to assess learning and memory. Biochemical parameters such as glutathione peroxidase and catalase activities, nitric oxide and malondialdehyde concentrations were measured to evaluate the antioxidant status in the rat hippocampus. Our results indicated that colchicine significantly impaired spatial memory and induced oxidative stress; in contrast, oleuropein pretreatment significantly improved learning and memory retention, and attenuated the oxidative damage. The results clearly indicate that oleuropein has neuroprotective effects against colchicine-induced cognitive dysfunction and oxidative damage in rats.