Phellinus merrillii extracts induce apoptosis of vascular smooth muscle cells via intrinsic and extrinsic pathways

Restenosis frequently occurs after balloon angioplasty. Percutaneous coronary intervention (PCI)‐induced artery damage is a significant part of triggering restenosis of the vascular smooth muscles (VSMC). This study aimed to study how ethanol extract of Phellinus merrillii (EPM) affected balloon inj...

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Bibliographic Details
Published in:Food science & nutrition 2023-12, Vol.11 (12), p.7900-7909
Main Authors: Chou, Pei‐Yu, Lu, Ya‐Ting, Sheu, Ming‐Jyh
Format: Article
Language:English
Subjects:
Angioplasty
Annexin V
Antibodies
Antioxidants
Apoptosis
Atherosclerosis
Balloon treatment
balloon‐injury‐induced neointima formation
Cardiovascular disease
Carotid arteries
Caspase-3
Cytotoxicity
Drug dosages
Ethanol
Fetal alcohol syndrome
GTP-binding protein
Iodides
Muscles
Mushrooms
Original
p53 Protein
Phellinus
Phellinus merrillii
Propidium iodide
Proteins
Restenosis
Smooth muscle
Stents
Western blotting
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Summary:Restenosis frequently occurs after balloon angioplasty. Percutaneous coronary intervention (PCI)‐induced artery damage is a significant part of triggering restenosis of the vascular smooth muscles (VSMC). This study aimed to study how ethanol extract of Phellinus merrillii (EPM) affected balloon injury‐induced overgrowth of VSMC, indicating neointima formation. Firstly, our results demonstrated that EPM notably decreased VSMC viability. A fragmentation assay and Annexin V/Propidium Iodide apoptosis assay showed that higher doses of EPM significantly induced the apoptosis of VSMC after 24 h of exposure. Total protein extracted from VSMC treated with EPM in various time and concentration periods was then conducted in Western blotting analysis. Our data demonstrated that EPM substantially elevated the p53, p21, Fas, Bax, p‐p38, and active caspase‐3 protein expressions. The results indicated that EPM induces VSMC apoptosis via intrinsic and extrinsic pathways. Also, our results demonstrated that EPM effectively attenuated the balloon injury‐induced neointima formation. In conclusion, the information offers a mechanism of EPM in inducing the VSMC apoptosis, thus as a potential interference for restenosis. Our results demonstrate that EPM induces the apoptosis of VSMCs by intrinsic and extrinsic apoptotic pathways. As well, we demonstrated that EPM effectively attenuated the neointima formation after balloon injury in animal studies.
ISSN:2048-7177
2048-7177
DOI:10.1002/fsn3.3707