Naringenin prevents non‐alcoholic steatohepatitis by modulating the host metabolome and intestinal microbiome in MCD diet‐fed mice

• Published in: Food science & nutrition 2023-12, Vol.11 (12), p.7826-7840
• Main Authors: Cao, Peng, Yue, Ming, Cheng, Yuanlei, Sullivan, Mitchell A., Chen, Wen, Yu, Huifan, Li, Fei, Wu, Sanlan, Lv, Yongning, Zhai, Xuejia, Zhang, Yu
• Format: Article
• Language: English
• Subjects: Alanine; Alanine transaminase; Aspartate transaminase; Bacteria; Carnitine; Cholesterol; Choline; Diet; Digestive system; Fatty liver; Gastrointestinal tract; Glutathione; gut microbiota; Inflammation; Intestinal microflora; Lipids; Liver; Liver diseases; Metabolic disorders; Metabolites; Metabolomics; Methionine; Microbiomes; Microbiota; Microorganisms; multi‐omics; Naringenin; NASH; Natural products; Nutrient deficiency; Original; Relative abundance; Triglycerides; Tyrosine
• ISSN: 2048-7177; 2048-7177
• DOI: 10.1002/fsn3.3700

Non‐alcoholic steatohepatitis (NASH) is a severe inflammatory phase of the non‐alcoholic fatty liver disease (NAFLD) spectrum and can progress to advanced stages of NAFLD if left untreated. This study uses multi‐omics data to elucidate the underlying mechanism of naringenin's reported benefit in alleviating (NASH). Male mice were fed a NASH‐inducing (methionine‐choline‐deficient) MCD diet with or without naringenin supplementation for 6 weeks. Naringenin prevented NASH‐induced histopathological liver damage and reversed the abnormal levels of hepatic triglyceride (TG)/total cholesterol (TC), serum TG/TC, serum alanine aminotransferase/aspartate transaminase, and hepatic malondialdehyde and glutathione. Importantly, naringenin intervention significantly modulated the relative abundance of gut microbiota and the host metabolomic profile. We detected more than 700 metabolites in the serum and found that the gut genus levels of Anaeroplasma and the [Eubacterium] nodatum group were closely associated with xanthine, 2‐picoline, and securinine, respectively. Tuzzerella alterations showed the highest number of associations with host endogenous metabolites such as FAHFA (8:0/10:0), FFA (20:2), carnitine C8:1, tridecanedioic acid, securinine, acetylvaline, DL‐O‐tyrosine, and Phe‐Asn. This study indicates that the interplay between host serum metabolites and gut microbiota may contribute to the therapeutic effect of naringenin against NASH. This study provides mechanistic insights into how naringenin suppresses NASH development. Naringenin intervention substantially restored the serum metabolome and gut microbiome in a NASH setting. Through the integration of multi‐omics data and correlation analysis, we found that the genera levels of Anaeroplasma, [Eubacterium]_nodatum_group, and Tuzzerella were significantly altered by naringenin treatment and closely correlated with 17 kinds of host serum endogenous metabolites, such as fatty acyl and amino acid derivatives.