Expression of the bglH gene of Lactobacillus plantarum is controlled by carbon catabolite repression

A newly identified bglH gene coding for a phospho-beta-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. Th...

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Bibliographic Details
Published in:Journal of bacteriology 1998-07, Vol.180 (13), p.3400-3404
Main Authors: Marasco, R, Muscariello, L, Varcamonti, M, De Felice, M, Sacco, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria
Bacteriology
Base Sequence
Benzyl Alcohols - metabolism
Cloning, Molecular
Consensus Sequence
Deoxyribonucleic acid
DNA
Enzyme Repression
Genes
Genes, Bacterial
Genetics and Molecular Biology
Glucose - metabolism
Glucosidases - biosynthesis
Glucosidases - chemistry
Glucosidases - genetics
Glucosides
Lactobacillus - genetics
Lactobacillus - growth & development
Lactobacillus - metabolism
Molecular Sequence Data
Open Reading Frames
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
RNA, Messenger - biosynthesis
Sequence Alignment
Sequence Homology, Amino Acid
Transcription, Genetic
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Summary:A newly identified bglH gene coding for a phospho-beta-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. The bglH gene was shown to be expressed on a monocistronic transcriptional unit. Its transcription was repressed 10-fold in L. plantarum cells grown on glucose compared to the beta-glucoside salicin as a sole carbon source. A catabolite-responsive element (CRE) spanning from -3 to +11 with respect to the transcriptional start point was found, and its functionality was assessed by mutational analysis. In vitro and in vivo DNA binding experiments suggested the occurrence of a DNA-protein complex at the CRE site, which would mediate glucose repression of bglH expression.
ISSN:0021-9193
1098-5530
DOI:10.1128/JB.180.13.3400-3404.1998